原发性高血压患者血管紧张素转化酶基因多态性与血清蛋白水平的关系

Association between angiotensin-converting enzyme gene polymorphism and serum levels in patients with essential hypertension

目的：探讨新疆地区维吾尔族、汉族原发性高血压（EH）患者血管紧张素转化酶（ACE）基因多态性与血清ACE水平的关系。方法采用随机抽样的方法，收集2012年8月至2014年1月新疆维吾尔自治区喀什地区疏附县人民医院就诊的EH患者104例（EH组），以同期该院门诊健康体检者102例作为对照（NT组），聚合酶链反应（PCR）检测ACE基因16号内含子I/D多态性，紫外分光光度法测定血清ACE水平，多重线性回归分析ACE基因型与血清ACE水平的关系。结果在维吾尔族、汉族人群中EH组血清ACE水平均高于NT组[维吾尔族：（45.21±14.53）U/L比（29.05±8.42）U/L，汉族：（46.42±16.45）U/L比（42.27±12.84）U/L，均P〈0.05]；EH组和NT组中不同ACE基因型之间血清ACE水平比较，差异均有统计学意义（均P〈0.05），且均为DD基因型〉DI基因型〉II基因型（均P〈0.05）。多重线性回归分析显示，维吾尔族和汉族人群中DD基因型、DI基因型相对于II基因型均能显著增加血清ACE水平（β=25.677、9.072，均P〈0.05）。结论新疆地区维吾尔族、汉族人群中ACE基因多态性与血清ACE水平有关联。

Objective To investigate the association between angiotensin-converting enzyme （ACE）gene polymorphism and serum ACE levels in patients with essential hypertension（EH）among Uygur and Han individuals in Xinjiang. Methods By using a random sampling method,104 EH patients treated in Shufu County People＇s Hospital,Kashgar of Xinjiang Uygur Autonomous Region between August 2012 and January 2014 were included in this study as the EH group,and a contemporary cohort of 102 healthy people who visited our clinic for routine checkup as normotensive controls（NT group）. Polymerase chain reaction （PCR）was used to detect I/D polymorphism of intron 16 on ACE gene. Ultraviolet spectrophotometry was used to measure the serum ACE level. Multiple linear regression was performed to analyze the relationship between ACE genotypes and serum ACE levels. Results Serum ACE levels in Uygur and Han EH patients were higher than those in the NT group[Uygur：（45.21 ± 14.53）U/L vs（29.05 ± 8.42）U/L,Han：（46.42 ± 16.45）U/L vs（42.27 ± 12.84）U/L,both P〈0.05]. In either EH or NT group,the serum ACE level varied across ACE genotypes with statistically significant difference （both P〈0.05）,and was highest in genotype DD,followed by DI and II genotypes（all P〈0.05）. Multiple linear regression analysis showed that the DD and DI genotypes was associated with significantly elevated serum ACE level compared with the II genotype in Uygur and Han individuals（β=25.677 and 9.072,both P〈0.05）. Conclusion ACE gene polymorphism is associated with serum ACE level in Uygur and Han individuals in Xinjiang region.