黄腐酸对盐胁迫下红花种子萌发及幼苗生理特性的影响

Effect of Fulvic Aicid on Seed Germination and Seedling Physiological Characters of Carthamus tinctorius L. under Salt Stress

为探讨黄腐酸(FA)缓解红花受盐胁迫伤害的机制,以蒸馏水浸种为空白对照,研究盐(Na Cl)胁迫下红花种子的萌发情况以及不同FA浓度对盐胁迫下红花种子萌发及其幼苗生长的影响。结果显示,在Na Cl质量浓度≥60 mg/m L时,红花种子的发芽率显著降低,其中,当Na Cl质量浓度为120 mg/m L时,红花种子发芽率为19%;盐胁迫下适宜浓度的FA处理能显著提高红花种子的发芽率,降低幼苗叶片的MDA含量,促进叶绿素合成,增强幼苗SOD、POD、CAT活性,同时能增加细胞膜结构的稳定性。因此,盐胁迫能明显抑制红花种子的萌发及幼苗生长,采用适宜浓度的FA处理能够有效缓解盐胁迫对红花种子萌发及幼苗生长的伤害,促进红花种子萌发,提高红花幼苗的抗盐性。

The response mechanisms for FA to ease the injury of salt stress on Carthamus tinctorius L.were discussed in this paper. Using seeds soaked with distilled water as CK,the experiments tested seed germination under Na Cl stress,and the effect of different FA concentrations on seed germination and seedling growth. The results showed that when Na Cl concentration was higher than 60 mg / m L,seed germination rate was significantly reduced; when Na Cl concentration was higher than 120 mg / m L,seed germination rate was 19%. Under salt stress condition,treatment of appropriate concentration of FA could significantly improve seed germination rate,reduce content of MDA in the seedlings,increase the content of chlorophyll in the seedlings slowly,enhance enzyme activity of SOD,POD and CAT in the seedlings,and improve cell membrane stability. The conclusion is that salt stress significantly inhibit seed germination and seedling growth; appropriate concentrations of FA can effectively alleviate the injury of salt stress on seed germination and seedling growth of Carthamus tinctorius L.,and enhance seed germination and salt resistance of the seedlings.