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新型SPECT/MRI双模态显像剂SPION-DMSA-RGD-99Tcm的合成与生物评价 被引量：2

Synthesis and Biological Evaluation of SPECT/MRI Dual-modal Imaging Agent SPION-DMSA-RGD-^(99)Tc^m
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摘要 为制备纳米材料SPION-DMSA-RGD及其标记物SPION-DMSA-RGD-99 Tcm,探讨该标记物作为SPECT/MRI双模态显像剂的可能性,在水溶性纳米颗粒SPION-DMSA上连接c(RGDfC),得到了SPION-DMSA-RGD,并进行了结构表征。用99 Tcm对SPION-DMSA-RGD进行标记,并对该标记物进行了正常鼠和荷U87MG人脑神经胶质瘤裸鼠的生物分布研究,及荷U87MG人脑神经胶质瘤裸鼠的MRI和SPECT显像研究。研究结果表明,SPION-DMSA-RGD具有超顺磁性,99 Tcm标记率约为98%。正常鼠和荷U87MG人脑神经胶质瘤裸鼠的生物分布结果表明,SPION-DMSA-RGD-99 Tcm在血液中清除较快,在肝脏中摄取较高,在肿瘤中有摄取。荷U87MG人脑神经胶质瘤裸鼠的MRI和SPECT显像结果表明,SPION-DMSA-RGD和SPION-DMSA-RGD-99 Tcm的肿瘤主动靶向作用明显。以上结果提示,对于荷U87MG人脑神经胶质瘤裸鼠,SPION-DMSA-RGD-99 Tc m是一种SPECT/MRI双模态显像剂。 In order to investigate the possibility of SPION-DMSA-RGD-99 Tcm as a SPECT/MRI dual-modal imaging agent,SPION-DMSA-RGD and its radiolabeled SPION-DMSA-RGD-99 Tcm were prepared.SPION-DMSA-RGD was prepared based on SPION-DMSA and c（RGDfC）.SPION-DMSA-RGD was characterized by means of various methods.SPION-DMSA-RGD-99 Tcm was given by labeling with 99 Tcm.The biodistribution studies of SPION-DMSA-RGD-99 Tcmin normal mice and the nude mice bearing U87 MG human glioma were performed.The MRI and SPECT imaging of SPION-DMSA-RGD and SPION-DMSA-RGD-99 Tcm in the nude mice bearing U87 MG human glioma were carried out,respectively.The results show that SPION-DMSA-RGD exhibits super-magnetic properties.The labeling yield of SPION-DMSA-RGD with 99 Tcm is about 98%.SPION-DMSA-RGD-99 Tcm displays fast clearance from the blood and high accumulation in the liver.The tumor uptake of SPION-DMSA-RGD-99 Tcmis higher than that of SPION-DMSA-99 Tcm.The MRI and SPECT images show that the tumor active targeting of SPION-DMSA-RGD and SPION-DMSA-RGD-99 Tcm are obvious.Therefore,SPION-DMSA-RGD-99 Tcm can be acting as a SPECT/MRI dual-modal imaging agent for nude mice bearing U87 MG human glioma.
作者 王晓 崔海平 史旭东 梁积新 孙钰林 申一鸣 沈浪涛
机构地区 中国原子能科学研究院国家同位素工程技术研究中心 原子高科股份有限公司
出处 《原子能科学技术》 EI CAS CSCD 北大核心 2015年第11期1932-1938,共7页 Atomic Energy Science and Technology
关键词 超顺磁氧化铁纳米颗粒 二巯基丁二酸 c(RGDfC) 99 Tcm SPECT/MRI双模态显像剂 super-magnetic iron oxide nanoparticle DMSA c（RGDfC） 99 Tcm SPECT/MRI dual-modal imaging agent
分类号 O615.4 [理学—无机化学]
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参考文献18

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原子能科学技术
2015年 第11期

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