Anti--inflammatory effect of ethyl acetate extract of Sedum aizoon L. in LPS-stimulated RAW 264.7 macrophages and its HPLC fingerprint

基于炎症细胞模型的景天三七醋酸乙酯部位抗炎活性及其HPLC指纹图谱的研究（英文）

Sedum aizoon L.（SA） has been widely used for treatment of various hemorrhages, insomnia, pain and trauma？ however, its anti-inflammatory activity is unknown. In this study, we firstly investigated the anti--inflammatory activity of SA extracts by petroleum ether（PE）, ethyl acetate（EtOAc）, and H2O in LPS-stimulated RAW 264.7 cells. Results showed that the EtOAc extract rich in phenolics and flavonoids significantly inhibited LPS-induced NO, TNF--α, and IL-6 production in RAW 264.7 cells（P0.01）, suggesting that this extract possessed potent anti--inflammatory activity. The phytochemical profile of the effective extract was subsequently analyzed by HPLC fingerprint with 11 standards. The results indicated that gallic acid, protocatechuic acid, p--hydroxybenzoic acid, ethyl gallate, iriflophene, 5,7-dihydroxy chromone, quercitrin, quercetin, luteolin, kaempferol, and isorhamnetin were present in this extract, which might contribute to its anti-inflammatory activity. Thus, the Et -OAc extract of SA showed anti-inflammatory activity and could be used as a potential natural anti--inflammatory agent.

景天三七广泛用于治疗各种出血、失眠、创伤肿痛,但关于该药抗炎活性尚未见报道。本研究的目的是基于炎症细胞模型探讨景天三七提取物包括石油醚、醋酸乙酯及水部位的抗炎活性。结果显示,富含酚酸和黄酮类成分的景天三七醋酸乙酯部位能显著抑制脂多糖诱导的RAW 264.7细胞中NO,TNF--α,IL--6的释放(P<0.01),表明其醋酸乙酯部位具有潜在的抗炎活性,同时建立该抗炎活性部位的HPLC指纹图谱。HPLC指纹图谱分析显示其抗炎活性可能与其所含的没食子酸、原儿茶酸、对羟基苯甲酸、没食子酸乙酯、鸢尾酚酮、5,7--二羟基色原酮、槲皮苷、槲皮素、木犀草素、山奈酚和异鼠李素等成分有关。综上,景天三七醋酸乙酯部位体外具有抗炎活性,是一潜在的天然抗炎剂。