表面增强拉曼光谱法对3种β受体激动剂化合物的定性和定量检测

Qualitative and Quantitative Determination of 3 Kinds of β Agonist Molecules by Surface Enhanced Raman Spectrometry

目的以硫酸特布他林、盐酸班布特罗与盐酸氯丙那林为例,研究并建立利用表面增强拉曼光谱检测β受体激动剂的定性与定量方法。方法选择800~850,1 050,1 450 cm^-1处的3个特征峰作为快速鉴定样品中β受体激动剂类药物的定性依据,并选择盐酸氯丙那林为代表,在800~850 cm^-1信号处进行定量检测。结果盐酸氯丙那林在1~9 mg·L^-1内线性关系良好,相关系数为R2=0.997 2,最低检出限为0.1 mg·L^-1。结论本法迅速简便,可实现无损检测,确证性高。

OBJECTIVE To study and establish the method for determination of β agonists in human urine by surface enhanced Raman spectrometry, taking clorprenaline hydrochloride, bambuterol hydrochloride and clorprenaline hydrochloride as exemplars. METHORDS The peaks at 800^-850, 1 050 and 1 450 cm^-1 were identified as β agonist characteristics, while the assay was carried out taking clorprenaline hydrochloride at the peak 800^-850 cm^-1. RESULTS The calibration curve of dorprenaline hydrochloride was linear in the range of 1^-9 mg·L^-1, with the correlative coefficient R2=0.997 2. The limit of detection was 0.1 mg·L^-1. CONCLUSION The method is proved to be rapid, simple, non-invasive and authentic.