HPLC测定银花芒果片中绿原酸、芒果苷和橙皮苷的含量

Determination of Chlorogenic Acid, Mangiferin and Hesperidin in Yinhua Mangguo Tablets by HPLC

目的建立HPLC同时测定银花芒果片中绿原酸、芒果苷和橙皮苷的含量。方法采用Waters Sun FireTM C18（4.6 mm×150 mm,5μm）色谱柱;流动相为甲醇（A）-0.2%磷酸水溶液（B）,梯度洗脱,流速为1.0 m L·min？1,检测波长为327 nm（0~10 min,绿原酸）,258 nm（10~20 min,芒果苷）,283 nm（20~40 min,橙皮苷）。结果绿原酸进样量在0.140~2.516μg内线性关系良好（r=0.999 9,n=6）,平均回收率为99.4%,RSD为1.2%（n=9）;芒果苷进样量在0.042~0.761μg内线性关系良好（r=0.999 9,n=6）,平均回收率为99.9%,RSD为1.4%（n=9）。橙皮苷进样量在0.076~1.361μg内线性关系良好（r=0.999 8,n=6）,平均回收率为99.9%,RSD为0.7%（n=9）。结论该方法简便、快捷、准确,可用于该制剂的质量控制。

OBJECTIVE To establish an HPLC method for determination of chlorogenic acid, mangiferin and hesperidin in Yinhua Mangguo tablets. METHODS Waters Sun FireTM C18（4.6 mm×150 mm, 5 μm） column was used. The mobile phase was methanol-water containing 0.2% phosphoric acid with gradient elution mode at the flow rate of 1.0 m L·min？1; the detection wavelength was 327 nm（0？10 min） for chlorogenic acid, 258 nm（10？20 min） for mangiferin, 283 nm（20？40 min） for hesperidin. RESULTS Chlorogenic acid had a good linearity in the range of 0.140？2.516 μg, the average recovery was 99.4% with RSD of 1.2%（n=9）. Mangiferin had a good linearity in the range of 0.042？0.761 μg, the average recovery was 99.9% with RSD of 1.4%（n=9）. Hesperidin had a good linearity in the range of 0.076？1.361 μg, the average recovery was 99.9% with RSD of 0.7%（n=9）. CONCLUSION The method is simple, accurate and reproducible. It can be used to determine chlorogenic acid, mangiferin and hesperidin in Yinhua Mangguo tablets.