铁皮石斛白绢病菌的分离鉴定与抑菌药剂筛选

Isolation of Pathogen and Fungicide Selection for Sclerotial Blight in Dendrobium officinale

通过对分离到的铁皮石斛白绢病菌进行形态特征观察、致病性测定及rDNA-ITS的同源性分析,确定侵染铁皮石斛的病原菌为Sclerotium rolfsii Sacc.(有性态为Athelia rolfsii),与罗氏阿太菌(Athelia rolfsii,登录号:HQ420816.1)同源性达到100%。选择5种药剂多利维生、80%乙蒜素、三唑酮、百菌清、奥力克-霉止,采用菌丝生长速率法,对白绢病菌进行室内抑菌测定。经室内抑菌试验及方差分析结果表明,多利维生与80%乙蒜素抑制率均可达87%以上,与对照相比达极显著水平。

The pathogen that causedSclerotialblight in Dendrobium officinaleKimura et Migo was identified to beSclerotiumrolfsii Sacc.by its morphological characteristics,as well as through the pathogenicity test and sequence homology analysis of its rDNA-ITS.The ITS sequence of the funguswas 100% identical with that of Atheliarolfsii（GenBank ID：HQ420816.1）.Subsequently,a toxicity test on 5fungicides for controlling S.rolfsii was conducted in the laboratory by using mycelium growth rate method and variance analysis.The results showed thatpolyversum and 80% ethylicin hada significant effect on controlling the pathogen with aninhibitory rate exceeding 87%,which reached an extremely significant level.