表皮生长因子与生长抑素在急性酒精中毒大鼠颌下腺表达的变化

Expression of epidermal growth factor and somatostatin in submandibular glands of rats with acute alcohol intoxication

目的:观察表皮生长因子(epidermal growth factor,EGF)、生长抑素(somatostatin,SS)在急性酒精中毒大鼠颌下腺表达的变化.方法:正常♂SD大鼠36只,按配对原则随机分为正常对照组(normal control group,NCG)(n=12)及实验组(experiment group,EG)(n=24).实验组大鼠予53度白酒按10 g/kg的剂量灌胃,并于灌胃后0.5、1.0、1.5、2.0 h取其颌下腺,用免疫组织化学SABC法及图像分析方法观察EGF、SS的表达变化.结果:与正常对照组比较,在急性酒精中毒期间,颌下腺EGF、SS阳性细胞数量增加,自灌胃后1.5 h始有统计学意义,尤以灌胃后2.0 h最为明显(正常对照组vs实验2.0 h组:50.56±4.21 vs 62.99±6.59,F=8.297,P<0.05;43.29±4.88 v s 53.88±7.63,F=9.161,P<0.05);平均灰度值逐渐下降,自灌胃后1.5 h始有统计学意义,以2.0 h最为显著(正常对照组vs实验2.0 h组:169.38±7.88 vs156.92±6.81,F=28.137,P<0.05;156.97±12.24 vs 148.31±7.41,F=20.387,P<0.05).结论:急性酒精中毒对大鼠颌下腺EGF、SS的合成和分泌有明显影响.

AIM：To detect the expression of epidermal growth factor（EGF） and somatostatin（SS）in submandibular glands of rats with acute alcohol intoxication.METHODS：Male rats（n = 36） were divided into a normal control group（NCG,n- 12） and an experiment group（EG,n = 24）.The EG was intragastrically given 53%alcohol.Submandibular gland tissue samples were taken from each group at 0.5,1.0,1.5,and 2.0 h to detect the expression of EGF and SS by immunohistochemistry.RESULTS：As compared with the NCG,the numbers of EGF and SS positive cells increased during the period of acute alcohol intoxication,which were statistically significant since 1.5 h,especially prominent at 2.0 h（NCG vs EG 2 h：50.56 ± 4.21 vs 62.99 ± 6.59,F = 8.297,P 0.05;43.29 ± 4.88 vs 53.88 ± 7.63,F = 9.161,P 0.05）;the mean grey values decreased gradually,which were statistically significant since 1.5 h,especially prominent at 2.0 h（NCG vs EG 2 h：169.38 ± 7.88 vs 156.92 ± 6.81,F = 28.137,P 0.05;156.97 ± 12.24 vs 148.31 ± 7.41,F = 20.387,P 0.05）.CONCLUSION：Acute alcohol intoxication alters EGF and SS which are synthetized and secreted by the submandibular glands.