HPLC法测黄瓜籽中芸香苷、异槲皮苷含量

Determination of Isoquercitrin and Rutin in Cucumber Seeds by HPLC

建立HPLC双波长法同时测定黄瓜籽中芸香苷、异槲皮苷含量的方法。以shim-pack VP-ODS C18(250 mm×4.6 mm,5μm)为固定相,以乙腈-0.2%磷酸溶液(15∶85)为流动相,流速1.0 m L/min,检测波长为254、273 nm,柱温30℃。芸香苷、异槲皮苷分别在0.001 59 mg/m L^0.159 mg/m L(r=0.999 8)和0.001 06 mg/m L^0.106 mg/m L(r=0.999 5)线性范围内与峰面积呈良好的线性关系,平均回收率分别为99.24%(RSD=0.4%,n=6)和98.56%(RSD=0.5%,n=6)。该方法快速、简便、准确、可靠。

To set up an HPLC method of double-wavelength simultaneous determination of the content for rutin and isoquercitrin in Cucumber seeds. The solid phase was shim-pack VP-DOS C18 （250 mm×4.6 mm,5μm）. The mobile phase was composed of acetonitrile-0.2%Phosphoric acid （15∶85）, the flow rate was 1.0 mL/min, the detection wavelength was set at 254 nm and 273 nm ,the temperature of chromatographic column was 30℃. good linear relationships were found within the range of 0.001 59 mg/mL-0.159 mg/mL （r=0.999 8）of rutin and 0.001 06 mg/mL-0.106 mg/mL （r=0.999 5） of isoquercitrin the average of recovery rate was 99.24%（RSD=0.4%,n=6） and 98.56%（RSD=0.5%,n=6）. The results of the study showed that this method was rapid, simple, accurate and reliable.