抗菌脂肽分离纯化的研究

Research on isolation and purification of antimicrobial peptides

为分离、鉴定枯草芽孢杆菌S2株产生的抗菌脂肽类化合物主要成分种类和特性。本试验采用s2菌株的发酵液，经大孔吸附树脂XD吸附、洗脱，将产物与Surfactin（表面活性剂）标准品同时进行薄层色谱（TLC）分析，结果表明，S2菌株代谢产物主要为环状脂肽类化合物Surfactin。运用液质联用（LC—MS）分析技术对S2菌株产生的脂肽化合物Surfactin进行了分离，对其在代谢产物中的含量及结构进行了分析，并对其分子量进行了推测，结果显示，枯草芽孢杆菌S2菌株在发酵前期合成的抗茵脂肽类化合物Surfactin（表面活性剂）含量达77．39％，分子量主要分布在1036．6933～994．6449之间，通过研究进一步确定了S2菌株产生的代谢产物是SurfactinA、SurfactinB和SurfactinC的同系混合物。

For isolation and identification of S2 strains of bacillus subtilis producing fat antibacterial peptide compound main component types and features. Test using S2 strains fermented liquid, by macroporous adsorption resin adsorption and elution, XD product with Surfactin （surfactant） standard and thin layer chromatography （TLC） analysis. The results show that the S2 main strain metabolites in circular Surfactin fat peptide compounds;Using liquid mass combination （LC - MS） analysis technology of S2 strains produce fat Surfactin peptide compounds of separation and its metabolites in the content and structure are analyzed, and its molecular weight is speculated that, according to the results of S2 strains of bacillus subtilis in the early stage of the fermentation Surfactin synthetic fat antibacterial peptide compounds （surfactant） content was 77.39%, and the main distribution of molecular weight between 1 036.693 3 994.644 9, through further research to determine the metabolites produced by the strain S2 is Surfactin, Surfactin B and Surfactin C homologous mixture.