星形细胞瘤组织中生长抑制因子4和缺氧诱导因子-1α的表达及其临床意义

Expression and clinical significance of ING4 and HIF-1 alpha in brain astrocytoma

目的 探讨人脑星形细胞瘤中生长抑制因子（ING）4和缺氧诱导因子（HIF）-1o的mRNA表达情况及其与肿瘤恶性程度及病理学分级的关系.方法 应用逆转录酶聚合酶链反应（RT-PCR）方法检测郑州大学第二附属医院2009年1月至2010年6月45例Ⅰ-Ⅳ级的人脑星形细胞瘤标本及11例人非瘤脑组织中ING4和HIF-1 α的mRNA表达水平,并分析其相关性.结果 ING4在非瘤脑组织的mRNA相对表达量为1.19±0.22,在Ⅰ-Ⅳ级的人脑星形细胞瘤中的相对表达量分别为0.91±0.19、0.74±0.28、0.54±0.33及0.22±0.19,与对照组脑组织相比,星形细胞瘤中ING4基因的mRNA表达水平明显降低（P＜0.05）,且随着病理学级别的升高,表达逐渐降低.HIF-1α在非瘤脑组织的mRNA的表达量为0.26±0.16,在Ⅰ-Ⅳ级的人脑星形细胞瘤中表达量分别为0.34±0.19、0.50±0.23、0.96±0.15及1.04±0.15,星形细胞瘤中HIF-1 α基因的mRNA表达水平明显升高,且病理学级别越高,表达也越高（P＜0.05）.随着肿瘤恶性程度的增加,ING4与HIF-1α的mRNA表达呈负相关.结论 ING4和HIF-1α基因在人脑星形细胞瘤的发生和发展中发挥作用,并与星形细胞瘤的恶性程度密切相关.

Objective To study the mRNA expression level of growth inhibition factor 4 （ING4） and hypoxia inducing factor 1 alpha （HIF-1 alpha）, and their relationship with tumor malignant degree or the pathology classification in human brain astrocytoma.Methods The mRNA expression levels of ING4 and HIF-1 alpha were detected by RT-PCR method in 45 cases of grade Ⅰ-Ⅳ human brain astrocytoma and 11 cases of control brain tissues from January 2009 to June 2010 in the Second Affiliated Hospital of Zhengzhou University, and their correlation was also analyzed.Results In the non-tumor brain tissue, the expression level of ING4 mRNA was 1.19 ±0.22, while they were 0.91 ±0.19, 0.74 ±0.28, 0.54 ±0.33 and 0.22 ±0.19 in Ⅰ-Ⅳ grade astrocytoma, respectively.Compared with the non-tumor control, the mRNA expression level of ING4 gene decreased significantly in the astrocytoma （P 〈 0.05）.And the expression of ING4 gradually reduced with the increase of the pathological classification of the astrocytoma.In the nontumor brain tissue, the expression level of HIF-1 alpha mRNA was 0.26 ± 0.16, and they were 0.34 ± 0.19, 0.50 ±0.23, 0.96 ±0.15 and 1.04 ±0.15 in Ⅰ-Ⅳ grade astrocytoma, respectively.For HIF-1 alpha gene, the mRNA expression level increased significantly in the astrocytoma.Meanwhile, the expression gradually increased with the increase of the pathological classification of the astrocytoma （P 〈 0.05）.The mRNA expression showed a negative correlation between ING4 and HIF-1 alpha with the increase of the tumor malignant degree.Conclusion The ING4 and HIF-1 alpha genes play a role in the tumorigenesis and development of human brain astrocytoma, and closely associate with the malignant degree of astrocytoma.