补肾降压方对糖脂代谢异常的自发性高血压大鼠脂联素通路的影响

Effect of Tonifying Kidney Anti-Hypertension Decoction on Adiponectin Signaling Pathway in Spontaneously Hypertensive Rats with Abnormal Glucose and Lipid Metabolism

目的:观察补肾降压方对糖脂代谢异常的自发性高血压大鼠(spontaneous hypertensive rat,SHR)的影响及其对脂联素通路的影响。方法:选用清洁级5周龄SHR 40只,随机分为正常组和高糖高脂组。8周造模后将高糖高脂组大鼠分为模型组(空白SHR伴糖脂代谢异常),卡托组(卡托普利)和补肾组(补肾降压方)。连续用药8周。检测血压,血糖,血脂,胰岛素。心肌组织切片行HE染色观察心脏组织病理形态学改变。Elisa法检测血清中ADP的含量。实时定量PCR检测心脏组织和肝脏组织PPARα的表达。Western blot检测心脏AdipoR1、AdipoR2的蛋白表达。结果:与SHR组比较,模型组血压明显升高,卡托组和补肾组有明显降低;与模型组相比,卡托组和补肾组都有明显降低;与卡托组比较,补肾组有升高的趋势。与SHR组相比,模型组,卡托组,补肾组胰岛素均有降低;与模型组相比,卡托组和补肾组皆有降低的趋势。与SHR组相比,模型组,卡托组,补肾组脂联素均有降低趋势;与模型组相比,卡托组与补肾组有升高趋势。心脏PPAR-αmRNA表达中,模型组,卡托组和补肾组较SHR组有所升高;与模型组比较,卡托组和补肾组皆有所降低。在AdipoR1蛋白表达的比较中,模型组,卡托组,补肾组较SHR组均有升高;与模型组相比,卡托组与补肾组有降低趋势。在AdipoR2蛋白表达的比较中,模型组,卡托组,补肾组较SHR组均有升高,其中模型组和卡托组有显著性差异(P<0.05);与模型组和卡托组相比,补肾组有降低趋势。结论:补肾降压方对SHR伴糖脂代谢异常具有调节作用,对心脏具有一定保护作用,其作用很有可能通过调节脂联素通路实现。

Objective： To observe the effect of tonifying kidney anti-hypertension decoction on SHR with abnormal blood lipid and blood glucose and adiponectin pathway. Method： Totally 40 5-week-old clean grade SHRs were randomly divided into the normal diet group and high-fat group. After the modeling at the 8thweek,the high-fat group rats were divided into three groups： model group（ blank SHR with abnormal glucose and lipid metabolism）,captopril group and tonifying kidney group（ tonifying kidney anti-hypertension decoction）. After the treatment of 8 weeks,the rats were killed to test their blood pressure,glucose,blood lipid and insulin. Myocardial tissue sections were stained with HE to observe the pathological changes in heart. The content of ADP in serum was detected by Elisa assay. Result： Before the treatment with drugs,blood pressure of SHRs with a high sugar and fat diet had no statistical difference. Blood pressure of groups with a high sugar and fat diet was higher than those with normal diet（ P〈0. 05）. After the modeling at the 4thweek,compared with the model group,captopril group showed a lower blood pressure significantly（ P〈0. 05）. After 8 weeks,compared with the model group,captopril group and tonifying kidney group showed statistically significant difference in the blood pressure（ P〈0. 01）.Compared with SHR group,model group,captopril group,tonifying kidney group showed decreased adiponectin,and model group had significant difference（ P〈0. 05）. In PPAR- mRNA of heart,the model group,captopril group and tonifying kidney group were higher than SHR group. Compared with the model group,captopril group and tonifying kidney group were lower. The expressions of AdipoR1 protein in model group,captopril group and tonifying kidney group were higher than that in SHR group. Compared with the model group,captopril group and tonifying kidney group were lower. AdipoR2 protein expression in model group,captopril group and tonifying kidney group were higher than that in SHR group. Compared with the model group and the captopril group,tonifying kidney group had a decreasing tendency. Conclusion： Tonifying kidney anti-hypertension decoction can regulate the abnormal blood lipid and blood glucose of SHRs and protect their hearts. Its mechanism may be correlated with the regulation of adiponectin pathway.