基于均匀设计法评价瓜蒌与制川乌反药配伍组合的祛痰镇咳作用

Expectorant and Antitussive Effects of Combined Administration of Trichosanthis Fructus and Aconiti Radix Cocta Based on Uniform Design

目的:研究瓜蒌与制川乌不同配比和剂量变化对小鼠祛痰、镇咳作用的影响。方法:采用均匀设计法,按2因素7水平以小鼠气管酚红分泌量和咳嗽次数为指标,观察瓜蒌和制川乌合煎液口服给药后对小鼠祛痰、镇咳作用的影响,并选取有显著意义的配比和剂量进行验证。结果:对气管酚红分泌量的影响:合煎液多数配比能增加小鼠气管酚红分泌量;经回归分析可知,瓜蒌有祛痰作用而制川乌没有,瓜蒌与制川乌配伍存在拮抗作用,且瓜蒌-制川乌为1∶2时拮抗作用最大。对咳嗽次数的影响:合煎液所有配比均能降低咳嗽次数;经回归分析可知,瓜蒌与制川乌分别都有镇咳作用,但二者之间没有交互作用。结论:瓜蒌与制川乌配伍能拮抗瓜蒌的祛痰作用,但对镇咳效用没影响。

Objective： To study the influence of the combined administration of Trichosanthis Fructus and Aconiti Radix Cocta at different dose or ratio on thier expectorant and antitussive effects. Method： The uniform design method was used based on two factors and seven levels to investigate phenolsulfonphthalein excretion and number of cough in mice and the influence of the combined administration of Trichosanthis Fructus and Aconiti Radix Cocta at different dose or ratio on theirexpectorant and antitussive effects. A demonstration test was conducted by choosing significant ratio and dose. Result： Combined administration of Trichosanthis Fructus and Aconiti Radix Cocta at most ratios can increase the phenolsulfonphthalein excretion in mice. According to the regression analysis,Trichosanthis Fructus had an expectorant effect in mice. And the expectorant effect of the combined administrationof Trichosanthis Fructus and Aconiti Radix Cocta was not lower than that of the single administration of Trichosanthis Fructus in mice. At the rate of 1∶2 between Trichosanthis Fructus and Aconiti Radix Cocta,the expectorant effect of the combined administrationwas almost the worst. Both Trichosanthis Fructus and Aconiti Radix Cocta at all ratios decreased the times of cough. According to the regression analysis,the combined administration of Trichosanthis Fructus and Aconiti Radix Cocta had good antitussive effect,but they had not an interactive effect with each other.Conclusion： The combined administration of Trichosanthis Fructus and Aconiti Radix Cocta could decrease the expectorant effect of Trichosanthis Fructus,but had no effect on the antitussive effect of Trichosanthis Fructus.