贝酵母耐硫相关基因FZF1的克隆与分析

Cloning and sequence analysis of the FZF1 gene concerning sulfur tolerance from Saccharomyces bayanus

FZF1基因在酿酒酵母中对亚硫酸盐转运蛋白编码基因SSU1具有正向调节作用,但在贝酵母中尚无相关报道,本研究拟通过对该基因进行克隆和生物信息学分析为后续研究做出参考。首先对贝酵母FZF1基因进行克隆,并利用在线分析工具Prot Param、Prot Scale、TMHMM、Predict Protein、Swiss-Model等软件对其编码蛋白质的基本理化性质进行分析,同时预测了该基因所编码蛋白质的二级结构和三级结构。结果表明:该核苷酸序列含有一个长900 bp的开放阅读框,可编码299个氨基酸;编码的蛋白质为在细胞核中行使调控功能的亲水蛋白,含有18个丝氨酸(S)激酶潜在磷酸化位点、一个Coil区和4个锌指结构域,与酿酒酵母FZF1基因所编码的蛋白质结构和性质极为相似。可初步认为贝酵母FZF1基因与细胞的耐硫性相关;而贝酵母FZF1基因所编码的蛋白质中仅有4个锌指,则可能是贝酵母硫耐受能力比酿酒酵母弱的重要原因。

The FZF! gene of Saccharomyces cerevisiae was found to be a positive regulator of SSU1 transcription, but there was no report about the FZF1 gene of Saccharomyces bayanus.ln this study,the genes were cloned and the content of bioinformatics was analyzed by online tools for setting the foundation of the further research.Here the FZF1 gene of Sobayanus was cloned and the physical and chemical properties of FZF1 protein were analyzed by online analytical tools,such as ProtParam, ProtScale,TMHMM, PredictProtein and Swiss-Model.At the same time, the protein secondary structure and tertiary structures were predicted.The results indicated that the FZF1 gene contained an opening reading frame（ORF）of 900 bp encoding a 299 predicted amino acids.The protein was a hydrophilic protein exercising their control functions in the nucleus. It contained t8 serine kinase potential phosphorylation sites,a coil domain and 4 zinc finger domains.The protein encoded by FZF1 gene of S.cerevisiae was very similar with the predicted protein.So the FZF! gene of S.bayanus was inferred to be associated with the sulfur resistance.And the protein encoded by the FZF1 gene of S.bayanus only contains 4 zinc finger domains.it was one of the most important reasons that the sulfur tolerance of most S.bayanus was weaker than most S.cerevisiae.