双孢菇漆酶的固定化及其对邻苯二甲酸二甲酯降解的研究

Immobilization of laccase from Agaricus bisporus and the degradation of dimethyl phthalate

为提高漆酶的利用效率,进一步开发其在环境治理中的应用,以海藻酸钠为载体,采用单因素实验对双孢菇漆酶凝胶包埋固定化的工艺进行探讨,并利用固定化漆酶对邻苯二甲酸二甲酯(DMP)进行降解实验。结果表明,采用直接包埋方式,固定化最佳单因素条件为:给酶量160 U/g载体,海藻酸钠浓度为3%,CaCl_2浓度为0.15 mol/L,固定化时间为2 h,可以获得较佳的固定化效果。利用固定化漆酶降解DMP时,降解率随DMP初始浓度的增加而下降,在pH3、4.5对DMP具有较好的降解效果,在25 mg/L的DMP溶液(pH4.5)中添加小分子介质ABTS浓度达到0.3 mmol/L,投加2 U/g固定化小球反应24 h,降解率最高达到56.9%。研究结果为处理含DMP污染提供了一种有效的方法和理论基础。

In order to improve the utilization efficiency and explore the application of laccase in environmental governance,the immobilization process of the laccase from Agaricus bisporus in sodium alginate gel spheres and the degradation of dimethyl phthalate （DMP）using the immobilized enzyme were investigated by single-factor experiments.According to the research, the best efficiency of immobilization was obtained by using the direct embedding method when the enzyme dosage was 160 U/g carrier,the concentration of sodium alginate was 3%, the concentration of CaCl2 was 0.15 mol/L and the immobilized time was 2 h.The degradation rate of DMP was decreased with the improvement of the initial concentration of DMP.In a initial pH of 3 and 4.5 ,the DMP has a good degradation effect.At a initial DMP concentration of 25 mg/L（pH4.5） ,the degradation rate of DMP reached .56.9% within 24 h,with 2 U/g the amount of immobilized enzyme-ball and 0.3 mmol/L of ABTS as redox mediator added. The research provided an efficient way and theoretical basis for DMP waste treatment.