基于水溶性共轭聚合物的多色荧光体系的构建及生物医学应用

Preparation of water-soluble conjugated polymers based multi-color fluorescent systems for biomedical applications

共轭聚合物(CPs)具有强的光捕获能力和独特的荧光信号放大效应,已经被广泛地用于生物大分子的检测以及细胞及动物水平上的荧光成像.在过去的十几年里,为了满足多色荧光检测及成像的需要,科研工作者设计并发展了一系列基于共轭聚合物的荧光共振能量转移(CPs-FRET)的多色荧光体系.这类多色荧光体系具有荧光强度高、光稳定性好以及细胞毒性低的优点.利用这类体系在单波长的激发光激发下即可实现目标分子的多色荧光检测及多色荧光成像.本文阐述了CPs的FRET机制以及基于CPs-FRET的多色荧光体系的构建方法,同时介绍了近期这类体系在生化检测和细胞成像领域中的研究进展以及目前所存在的问题和未来的发展展望.

Conjugated polymers（CPs） have gained increasing attention as an optical platform for sensitive detection of biomacromolecules and fluorescent imaging in cellular and animal level due to its excellent light-harvesting and light-amplifying properties. Over the past decades, in order to meet the needs of multi-color fluorescent detection and imaging, researchers have designed and developed a series of multicolor fluorescent systems based on the fluorescence resonance energy transfer properties of conjugated polymers（CPs-FRET）. The advantages of these systems include high brightness, excellent photostability, and low cytotoxicity. Multicolor fluorescence encoded detection of target molecules and fluorescence imaging can be achieved using such systems under a single excitation wavelength. In this tutorial review, we describe the FRET mechanism of CPs and the preparation of CPs-FRET based multicolor fluorescent systems, provide a brief review of the recent research progress of such systems in biochemical detection and cell imaging, and discuss the current problems and future direction of such systems.