摘要
The present study was designed to investigate the effects of start codon of nosM on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon ofnosMwere constructed, and nosihcptide production of each mutant was analyzed and compared. Replacement of the start eodon of nosM significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.
The present study was designed to investigate the effects of start codon of nos M on the biosynthesis of nosiheptide. Target genes were amplified by overlap PCR. After homologous recombination to construct engineered strains, nosiheptide production was analyzed by HPLC. Three mutants with different start codon of nos M were constructed, and nosiheptide production of each mutant was analyzed and compared. Replacement of the start codon of nos M significantly decreased the production of nosiheptide. In conclusion, start codon usage could greatly affect the biosynthetic efficiency in the biosynthetic gene cluster of nosiheptide.
基金
supported by the grants from the"111"Project from the Ministry of Education of China and State Administration of Foreign Export Affairs of China(No.111-2-07)
the National Key Project on Science and Technology of China(No.2012ZX09103101-030&2012ZX09201101-012)
National Science Foundation of China(No.81172967)
the Doctoral Fund from the Ministry of Education of China(No.20110096110011)
the Science&Technology Pillar Program of Jiangsu Province(No.SBE201371217)
the Priority Academic Program Development of Jiangsu Higher Education Institutions
