柳州市2014年手足口病实验室检测结果分析

Analysis of the laboratory diagnosis results of hand-foot-mouth disease in 2014 in Liuzhou city

目的明确柳州地区手足口病（hand,foot and mouth disease,HFMD）病毒的主要感染病毒型别及流行特征,为手足口病防治提供科学依据。方法收集2014年1月至2014年12月6 667例疑似手足口病感染者。运用实时荧光定量PCR（real-time quantitative polymerase chain reaction,RT-q PCR）方法,对肠道病毒71型（enterovirus 71,EV71）和柯萨奇肠道病毒16型（coxsackievirus A16,Cox Al6）特异性进行核酸检测。结果 6 667例患者标本中,EV71阳性数为1 310例,阳性率为19.65%,Cox A16阳性数为858例,阳性率12.87%。EV71和Cox A16合并感染阳性数为11例,阳性率为0.16%。男性人群手足口病发病率高于女性,4月~9月份为发病高峰。结论柳州市2014年手足口病主要致病病毒为EV71、Cox A16,基因分型对手足口病临床诊治及疾病爆发的预防有一定指导意义。

Objective To study the virus subtypes of hand-foot-mouth disease（HFMD） and prevalent features so as to provide scientific basis for the surveillance and control of HFMD. Methods A total of 6 667 specimens were collected from January to December in 2014. Nucleic acid in genotype of enterovirus 71（EV71） and coxsackievirus 16（Cox A16） were detected by real-time quantitative PCR（RTq PCR）. Results 1 310 cases（19.65%）, 858 cases（12.87%） and 11 cases（0.16%） were positive for EV71,Cox A16 and EV71 combined with Cox A16, respectively. Male ＇s morbidity was higher than that of female ＇s,and peaks of incidence occurred from April to September. Conclusion EV71 and Cox A16 are the main pathogenic viruses of HFMD in Liuzhou in 2014. Genetic analysis has profound guiding significance for HFMD diagnosis and outbreaks prevention.