MTDH在肝细胞癌发展过程中的表达及意义

Significance of MTDH expression in the developing process of hepatocellular carcinoma

目的探讨异黏蛋白(MTDH)的表达与肝细胞癌(HCC)发生发展的关系及其意义。方法应用免疫组织化学方法检测89例肝癌及其癌旁肝组织,37例肝硬化和25例正常肝组织中MTDH蛋白以及CD34,血管内皮生长因子(VEGF),肿瘤转移抑制基因蛋白(nm23)、p53、p21、Ki-67的表达,并分析MTDH与临床病理参数以及系列免疫组化指标的相互关系。结果 HCC组MTDH的阳性率为57.3%,明显高于肝硬化组织的29.7%和正常肝组织的24.0%,差异均有统计学意义(P<0.05);MTDH的表达与肿瘤的T分级、远处转移、HBV感染、不同程度微血管密度、nm23以及Ki-67的表达密切相关(P<0.05);61例肝癌随访中,总平均复发时间为(57.841±3.025)个月,MTDH阳性表达组的平均复发时间为(47.791±4.226)个月,低于阴性表达组的(59.418±3.507)个月,但差异无统计学意义(P>0.05)。结论 MTDH可能是一个潜在的评估HCC进展和预后的新生物学标志物,对肝癌的侵袭转移、血管生成等起重要作用。

Objective To investigate the relationship between the expression of metadherin（MTDH） and the carcinogenesis of hepatocellular carcinoma（HCC） and its related significance. Methods Immunohistochemistry was adopted to detect the expression levels of MTDH, CD34, vascular endothelial growth factor（VEGF）, nm23, p53, p21 and Ki-67 in a cohort of 89 pairs of HCC tissues and their matched adjacent hepatic tissues, 37 cases of hepatocirrhosis tissues and 25 cases of normal hepatic tissues. Meanwhile, we analyzed the correlation between MTDH and several clinicopathological parameters as well as some immunohistochemical indexes. Results The positive rate of MTDH expression in HCC tissues（57.3%） was significantly higher than that in either hepatocirrhosis tissues（29.7%） or normal hepatic tissue（24.0%, P〈0.05）. The MTDH expression was closely related to T category, distant metastasis, HBV infection, microvessel density and the expression of nm23 and Ki-67（P〈0.05）. For the 61 cases of HCC which were followed up, the overall average recurrence time was（57.841±3.025） months, and no statistical significance was found between MTDH-positive group [（47.791 ± 4.226） months] and MTDH-negative group [（59.418 ± 3.507） months], P〈0.05. Conclusion MTDH might be implicated as a crucial part in invasion, metastasis and angiogenesis in HCC,which has the potential to be a novel biomarker for the assessment of the development and prognosis of HCC.