长泰砂仁愈伤组织的诱导与继代培养

Callus Induction and Subculture of Amomum villosum CV:Changtaisa

以长泰砂仁的笋芽、果实和试管苗叶片为外植体进行愈伤组织的诱导及继代培养研究。结果表明:果实最适合用0.2%Hg Cl2消毒12 min,笋芽消毒15 min;最佳愈伤组织诱导培养基为MS+6-BA 2.0 mg/L+2,4-D 2.0 mg/L,最适合的外植体为无菌的试管苗叶片,诱导率最高为94%。最合适愈伤组织增殖培养基为MS+6-BA 2.0 mg/L+2,4-D 3.0 mg/L,增殖系数为4.1。

The buds, fruit and young leaves of A momum villosum CV：Changtaisa were taken as explants.The effects of different concentrations and combinations of plant growth regulators on cullus induction and subculture were studied.The results showed that the best sterilization method for materials were to soak explants into 0.2% HgCl2 for 12 rain and 15 min;The best medium for callus formation was MS＋6-BA 2.0 mg/L＋2,4-D 2.0 mg/L, yong leaves of plantlet were best explants ;The best subculture medium was MS＋6-BA 2.0 mg/L＋2,4-D 3.0 mg/L, the multiplication coefficient was 4.1.