客观评价骨癌痛致鼠行走距离缩短的新方法

OBJECTIVE EVALUATION OF SHORTENED WALKING DISTANCE INDUCED BY BONE CANCER PAIN IN THE MOUSE

目的：探讨自主滚轮测试（Voluntary Wheel Running Test）在评估C57BL/6小鼠股骨癌痛模型癌痛行为学上的可行性。方法：选用体重18-20 g雄性C57BL/6小鼠20只,随机分为两组（n=10）,肿瘤组：骨髓腔内接种Lewis肺癌细胞2×106个;对照组：采用同样方法接种等容积D-Hank＇s液。术前三天起每日测定两组小鼠在滚轮内自由行走20 min的圈数,取第三天（即术前一天）的测量结果为基础值。术后第1、7、9、12、14、18、21天各组均进行自发痛行为学测试及自主滚轮测试。结果：自发抬足评分和抬足次数测试结果显示：实验组抬足评分降低,抬足次数延长。第12天开始实验组与对照组相比,差异有统计学意义（P〈0.05）;自发抬足时间和自主滚轮测试结果显示：实验组自发抬足时间延长,自主运动减少。术后第9天开始实验组与对照组相比,差异有统计学意义（P〈0.05）。结论：自主滚轮测试方法可用于评估癌痛小鼠的疼痛程度。

Objective： To evaluate the feasibility of using independent roller test（Voluntary wheel running test） in a mouse model of bone caner pain. Methods： Twenty male C57BL/6 mice weighing 18-20 g were randomly divided into two groups（n = 10 per group）： cancer cell inoculated group： 2×10^6 Lewis lung cells in 10 ul D-Hank＇s were injected into the left femur bone marrow cavity; the control group received injections of D-Hank＇s. Spontaneous foot lifting time and voluntary wheel running test were measured in alternative days after the experiment. Results： Mice in the cancer group displayed gradual development of pain behavior associated with the development of transplant tumor lesion. The spontaneous foot lifting score was significantly reduced, and the lifting frequency was significantly increased from day 12 after injection. The spontaneous foot lifting duration was significantly increased, and the voluntary wheel running was significantly reduced from day 9 after inoculation. Conclusion： The voluntary wheel running test can be used to evaluate the level of pain in a mouse model of bone cancer.