复发性阿弗它溃疡患者血清中Th1,Th2类因子表达及意义

The expression of Th1 and Th2 cytokines in serum of patients with recurrent aphthous ulcer

目的:探讨Th1/Th2平衡在复发性阿弗他溃疡(RAU)免疫发病机制中的作用及意义。方法:RAU患者35例(轻型25例重型10例)及健康对照24例采用流式细胞术(CBA)检测患者溃疡期血清中Th1类细胞因子(IFN-γ、TNF-α、IL-2)及Th2类细胞因子(IL-4、IL-6、IL-10)的表达水平分析其与RAU临床特征的关系。结果:在轻型RAU患者中,IL-4、IL-10表达水平明显高于正常组(P<0.05);在重型RAU患者中JFN-γ、IL-4、IL-10表达水平明显高于正常组(P<0.05);重型RAU患者IFN-γ、IL-10表达水平明显高于轻型组(P<0.05);IFN-γ、IL-4、IL-10表达水平与RAU病情严重程度正相关(P<0.05)。结论:RAU患者表现为Th2细胞占优势的免疫应答;其中IFN-γ、IL-4、IL-10表达水平与RAU病情严重程度有关。

Objective：To explore the possible role and clinical significance of Th1 and Th_2 class cytokines in the pathogenesis of recurrent aphthous ulcer（RAU）.Methods：35 cases with RAU,25 of minor RAU（MiRAU） and 10 of major RAU（MaRAU）,were enrolled and 24 healthy subjects served as the controls.The expression levels of Th1 type cytokines（IFN-γ,TNF-α,IL-2） and Th_2type cytokines（IL-4,IL-6,IL-10） in the serum of peripheral blood were investigated by flow cytometry CBA methods and their relationship with the clinical features of RAU was analyzed by SPSS 11.5.Results：The expression level of IL-4 and IL-10 in MiRAU group was higher than that in the controls（P〈0.05）,and the expression level of IFN-γ,IL-4 and IL-10 in MaRAU group was higher than that in the controls（P〈0.05）.The expression level of IFN-γ and IL-10 in MaRAU was higher than that in MiRAU.There was a positive correlation between the levels of IFN-γ,IL-4,IL-10 and the severity of RAU.Conclusion：Predominant Th2 immune response plays a role in RAU,and the level of IFN-γ,IL-4 and IL-10 is correlated with the severity of RAU.