牙周炎患者外周血中Th亚群及龈沟液内IL-17的检测

Th subsets in peripheral blood and IL-17 in gingival crevicular fluid of the patients with periodontitis

目的:探讨Th亚群以及IL-17在牙周炎发病中的作用。方法:用流式细胞术检测健康对照10例,慢性牙周炎(CP)11例,侵袭性牙周炎(AgP)8例患者外周血中辅助性T细胞亚群Th1、Th2、Th17的水平,ELISA法检测龈沟液(GCF)中IL-17的水平。结果:与正常对照组相比2组牙周炎患者外周血中Th1、Th17所占比例明显升高(P<0.01),Th2比例明显降低(P<0.01);龈沟液内IL-17浓度明显升高(P<0.01)。CP组IL-17浓度与PD和AL正相关(P=0.000),AgP组IL-17与PD正相关(P=0.007)。结论:牙周炎患者机体免疫功能降低,龈沟液中IL-17升高与牙周炎发生发展有关。

Objective：To study the correlation of Th subsets in peripheral blood（PB） and IL-17 in gingival crevicular fluid（GCF） in the patients with periodontitis.Methods：10 healthy controls,11 cases with chronic periodontitis（CP） and 8 cases with aggressive periodontitis（AgP） were included.Th1,Th2 and Thl7 in PB were measured by flowcytometry,IL-17 in GCF was examined by ELISA.Periodental indexes（PLI,PD,AL and BI） were measured.Results：In the CP group and the AgP group,Thl and Thl7 were significantly higher,Th2 was lower than those in the control group（P〈0.01）（between CP and AgP groups,P〈0.05）,and IL-17 in GCF were significantly higher（P〈0.01）（between CP and AgP groups,P〉0.05）.In group CP IL-17 leve in GCF was positively correlated with PD and AL（P =0.000）,in group AgP with PD（P =0.007）.Conclusion：Deficiency of immunologic function and increase of IL-17 in the GCF is related with the onset and development of periodontitis.