A型核纤层蛋白在腰椎退行性疾病中的表达及其意义

Expression and significance of type A lamin in lumbar spinal degenerative disease

目的初步探讨A型核纤层蛋白(Lamin A)在腰椎退行性疾病中的表达特点及意义。方法将74例腰椎退行性疾病患者分为腰椎间盘突出组(32例)及腰椎管狭窄组(42例),另设腰椎正常组(12例)作为对照。采用免疫组织化学检查(免疫组化)及蛋白免疫印迹法检测Lamin A在3组腰椎间盘组织标本中的表达情况。结果免疫组化显示Lamin A在3组的椎间盘细胞中均有表达,腰椎管狭窄组的Lamin A在椎间盘细胞中的表达较腰椎正常组及腰椎间盘突出组明显;蛋白免疫印迹法检测3组椎间盘的纤维环组织均有Lamin A的表达,腰椎管狭窄组Lamin A条带灰度值为3.55±0.16,腰椎间盘突出组为1.02±0.13,腰椎正常组为0.78±0.14,3组比较差异有统计学意义(F=14.326,P<0.01),腰椎管狭窄组与腰椎间盘突出组及腰椎正常组比较差异均有统计学意义(P均<0.001),腰椎间盘突出组与腰椎正常组比较差异尚未有统计学意义(P=0.134)。结论随着腰椎退行性疾病病程的发展,Lamin A的表达逐渐升高,其可能与腰椎退行性疾病的发生、发展有一定联系。

Objective To preliminarily explore the expression levels and clinical significance of type A lamin （lamin A）in lumbar spinal degenerative disease.Methods Seventy four patients diagnosed with lumbar spinal degenerative disease were divided into the lumbar disc herniation （n =32）and lumbar spinal stenosis groups （n =42）and healthy subjects were recruited into the control group （n =12）.The expression of lamin A in the lumbar tissue samples from three groups was assessed by immunohistochemical examination and western blotting.Results Immunohistochemical analysis revealed that lamin A was expressed in the interver-tebral disk cells from all three groups.The staining of lamin A in the tissue from lumbar spinal stenosis group was stronger than those from the other two groups.Western blot demonstrated that lamin A was expressed in the fibrous ring tissue of intervertebral disk from all three groups.In the lumbar spinal stenosis group,the expres-sion level of lamin A was 3.55 ±0.16（gray level）,1.02 ±0.13 in the lumbar disc herniation group and 0.78 ± 0.14 in the control group with statistical significance among three groups （F =14.326,P 〈0.01）.Statistical significance was noted between the lumbar spinal stenosis and lumbar disc herniation group /the control group （both P 〈0.001）.There was no statistically significant difference between the lumbar disc herniation and con-trol groups （P =0.134）.Conclusions Over the progression of degenerative lumbar spinal disease,the ex-pression level of lamin A is gradually up-regulated,which is probably associated with the incidence and devel-opment of degenerative lumbar spinal disease.