基于明胶-碳纳米管构建癌胚抗原免疫传感器的研究

Study on construction of An immunosensor for carcinoembryonic based on carbon nanotube-gelatin composite

利用纳米金与明胶-碳纳米管（MWCNTs）复合物固载癌胚抗体制得较高灵敏度的电流型癌胚抗原（CEA）免疫传感器。首先将制得的明胶-碳纳米管复合物修饰在金电极表面成膜，再利用明胶中丰富的氨基吸附纳米金，并通过静电吸附作用吸附癌胚抗体（anti—CEA）。实验过程中采用循环伏安法对电极的修饰过程及响应性能进行表征。结果表明，制得的免疫传感器对癌胚抗原有良好的电流响应，其线性范围为0．1-80．0ng／mL，检出下限为0．03ng／mL。

A highly sensitive amperometric immunosensor for the determination of carcinoembryonic antigen （CEA） was eonstruted with the comprised of gelatin, muti-wall carbon nanotubes （MWCNTs）. At first, place the prepared gelatin-carbon nanotube composites on the gold electrode surface. Then, absorb the nano-Au by the numerous amino of gelatin and anti-CEA by electrostatic adsorption on its surface successively . In the experiment,the perparing process of the immunosensor was characterized by the cyclic vohammetrys. The results show that the immunosensor present highly sensitivity, with a linear range of 0.1 - 80.0 ng/mL and a detection limit of 0.03 ng/mL.