饲粮维生素B_6添加水平对3～5月龄生长獭兔消化代谢和抗氧化性能的影响

Effects of Dietary Vitamin B_6 Supplemental Level on Digestion and Metabolism,and Antioxidant Ability of 3 to 5-Month Old Growing Rex Rabbits

本试验旨在研究饲粮维生素B6添加水平对3-5月龄生长獭兔消化代谢和抗氧化性能的影响。选用200只3月龄獭兔,随机分成5组（每组40个重复,每个重复1只）,分别饲喂在基础饲粮中添加0、5、10、20和40 mg/kg维生素B6的试验饲粮。预试期7 d,正试期54 d。结果表明：饲粮维生素B6添加水平极显著影响食入氮（P=0.004 5）,显著影响可消化氮（P=0.034 4）和氮表观消化率（P=0.043 6）,其中10和20 mg/kg组食入氮极显著高于其他组（P〈0.01）,可消化氮和氮表观消化率均在10 mg/kg组达到最大值,但与20 mg/kg组差异不显著（P〉0.05）。饲粮维生素B6添加水平极显著影响蛋氨酸的表观消化率（P=0.006 8）,显著影响半胱氨酸（P=0.011 3）、谷氨酸（P=0.022 6）和丝氨酸的表观消化率（P=0.047 8）,并均在20 mg/kg组达到最大值。饲粮维生素B6添加水平显著影响胰腺胰蛋白酶（P=0.035 4）和糜蛋白酶活性（P=0.043 2）,并均在20 mg/kg组达到最高值。饲粮维生素B6添加水平显著影响血清总蛋白（P=0.014 3）和尿素氮含量（P=0.048 2）,并分别在20 mg/kg组达到最高值和最低值。饲粮维生素B6添加水平极显著影响血清总抗氧化能力（P=0.004 5）,显著影响血清谷胱甘肽过氧化物酶（P=0.044 2）和总超氧化物歧化酶活性（P=0.048 1）,并均在20 mg/kg组达到最高值,且血清丙二醛含量在20 mg/kg组达到最低值。综合本试验测定指标,3-5月龄生长獭兔饲粮适宜的维生素B6添加水平为10-20 mg/kg。

The experiment was conducted to investigate the effects of dietary vitamin B6 supplemental level on digestion and metabolism,and antioxidant ability of 3 to 5-month old growing Rex rabbits. A total 200 3-month old health Rex rabbits were randomly divided into 5 groups with 40 replicates in each group and 1 rabbit in each replicate. Rabbits in the 5 groups were fed the basal diet supplemented with 0,5,10,20 and 40 mg / kg vitamin B6,respectively. The trial lasted for 7 days for adaptation,and 54 days for test. The results showed as follows： dietary vitamin B6 supplemental level had an extremely significant influence on nitrogen intake（ IN）（ P = 0.004 5）,and had significant influences on digestible nitrogen（ DN）（ P = 0.034 4） and nitrogen apparent digestibility（ NAD）（ P = 0.043 6）. The IN in 10 and 20 mg / kg groups was extremely significantly higher than that in other groups（ P〈 0.01）,and the DN and NAD in 10 mg / kg group came to the maximum,but had no significant influences compared with 20 mg / kg group（ P〉 0.05）. Dietary vitamin B6 supplemental level had an extremely significant influence on methionine apparent digestibility（ P = 0.006 8）,had significant influence on cystine（ P = 0.011 3）,glutamate（ P = 0.022 6） and serine apparent digestibility（ P = 0.047 8）,and all of them in 20 mg / kg group came to the maximum. Dietary vitamin B6 supplemental level had significant influences on pancreatic trypsin（ P = 0. 035 4） and chymotrypsin activities（ P = 0. 043 2）,and the highest values were all found in the 20 mg / kg group. Dietary vitamin B6 supplemental level had significant influences on serum total protein（ TP）（ P = 0.014 3） and urea nitrogen（ UN） contents（ P = 0.048 2）,and the highest value of TP content and the lowest value of UN content were all found in the 20 mg / kg group. Dietary vitamin B6 supplemental level had an extremely significant influence on serum total antioxidant capacity（ T-AOC）（ P =0.006 9）,had significant influences on serum glutathione peroxidase（ GSH-Px）（ P = 0. 044 2） and total superoxide dismutase（ T-SOD） activities（ P = 0. 048 1）,and the highest values of them were all in the20 mg / kg group,meanwhile,the serum malonaldehyde（ MDA） content in the 20 mg / kg group had the minimum. Considering all indices of this experiment,the appropriate dietary vitamin B6 supplemental level is 10 to20 mg / kg for 3 to 5-month old growing Rex rabbits.