摘要
本研究建立了油桐属植物千年桐的AFLP分子标记技术体系,通过AFLP扩增筛选得到1对引物组合E7/M5,能够成功对千年桐的雌雄植株进行分子鉴别。AFLP分子标记技术得到的雄性特异性引物组合,扩增出大小为420 bp的雄性特异带,仅在雄性植株基因组DNA池中出现,而在雌性植株中未出现。本研究可为油桐属植物雌雄株的早期分子鉴别提供可靠的方法和依据。
Amplified Restriction Fragment Polymorphism marker technique was established and used to identify the sex of V.montana in this study. The primer combination of E7/M5 was screened by AFLP amplify to identify the male and female genomic DNA pool of V. montana. This marker generated a 420 bp male specific band, which was present in male plant and absent in all female plant. This marker provided reliable method and evidence in early sex identification of Vernicia at molecular level.
出处
《南方林业科学》
2015年第5期1-4,28,共5页
South China Forestry Science
基金
江西省青年科学基金计划项目"樟树5种化学型蛋白质组和转录组比较关联分析"(20133BAB21007)
江西省林业科学院财政专项重点项目"樟树基因组测序和基因组学分析"(2011511102)和"油桐
少叶黄杞及伯乐树苗木繁育及造林技术研究"(2011512201)
关键词
千年桐
AFLP
雌雄鉴别
Vernicia montana
AFLP
sex discrimination
