四君子汤对受照小鼠体重及外周血象的影响

Effects of Sijunzi decoction on body weight and peripheral blood in ^(60)Co γ irradiated mice

采用CCK-8试剂盒检测不同终浓度(0、5、25、50、250和500μg/m L)四君子汤及其单味组份作用48 h对AHH-1细胞的毒性作用,以及不同终浓度(0、0.4、2、10和50μg/m L)四君子汤及其单味组份预处理AHH-1细胞2 h后对经4 Gy60Coγ射线照射的细胞存活的影响。将BALB/c小鼠80只,雌雄各半,随机分为阴性对照组、照射对照组、四君子汤低剂量组(0.75 g/kg)、四君子汤中剂量组(2.25 g/kg)、四君子汤高剂量组(6.75 g/kg),检测各组照后3天和7天的外周血白细胞、红细胞、血红蛋白、血小板数量,以及照前7天至照后7天小鼠体重的变化,研究四君子汤对60Coγ射线照射引起的小鼠体重及外周血象的影响。结果显示,与对照细胞相比,5、25和50μg/m L四君子汤及其单味组份作用于AHH-1细胞48 h对细胞存活无明显影响(p>0.05),但50μg/m L四君子汤可显著提高受照后24 h AHH-1细胞的存活率(p<0.05)。四君子汤中剂量组小鼠外周血白细胞数与照射对照组相比显著升高(p<0.01),并且四君子汤中剂量组能明显减弱受照小鼠外周血血小板数的降低,同时四君子汤可以减轻辐射损伤所致的体重下降。上述研究结果表明,四君子汤对受照射AHH-1细胞具有一定的辐射损伤防护作用;四君子汤通过影响受照射后小鼠造血系统,促进体重恢复,减轻辐射所致损伤。

CCK- 8 Assay Kit was used to detect the survival rate of AHH- 1 cells treated by 0,5,25,50,250,500 μg/m L Sijunzi decoction and its single components for 48 h,or by 4 Gy γ- ray for 24 h after treatment with 0,0. 4,2,10,50 μg/m L Sijunzi decoction and its single components. A total of 80 BALB/c mice female/male were randomly divided into 5 groups： control group without treatment,irradiation group,Sijunzi low-dose group（ 0. 75 g/kg）,Sijunzi middle-dose group（ 2. 25 g/kg）,Sijunzi high-dose group（ 6. 75 g/kg）. WBC,RBC,HGB,PLT counts post-irradiation 3 days and 7 days were examined by blood cell analyzer. Body weights before and after irradiation were also observed to investigate the effect of Sijunzi decoction on 3. 5 Gy60 Co γ radiation-induced acute injury in mice. The results demonstrated that compared to control group cells（ 0 μg/m L）,the survival rate of AHH- 1 cells treated by 5,25 and 50 μg/m L Sijunzi decoction for 48 h was not changed（ p〉 0. 05）,but the survival rate of post-irradiation AHH- 1 cells increased at 50 μg/m L Sijunzi decoction（ p 〈0. 05）. After 3. 5 Gyγ- ray,the WBC count of middle-dose Sijunzi decoction group increased compared to radiation group（ p 〈0. 01）,moreover,the middle-dose Sijunzi decoction group can slow the PLT decline. Sijunzi groups also have good effect on the body weight regaining in mice after radiation. Sijunzi decoction had radiation-protective effect in post radiation AHH- 1 cells. In addition,Sijunzi reduced the injury in irradiated mice through hematopoietic system rebuilt and body weight regain.