摘要
经70%乙醇冷浸提取、不同溶剂萃取,在清除1,1-二苯基-2-三硝基苯肼(1,1-dipHenyl-2-picrylhydrazyl,DPPH)自由基活性跟踪下,通过多次柱层析及薄层层析(thin layer chromatograpHy,TLC),从药桑枝条中分离得到一种稀有黄酮类化合物,经波谱鉴定、结构分析确定其为桑根酮M(sanggenon M)。以人工合成的抗氧化剂二丁基羟基甲苯(butylated hydroxytoluene,BHT)为对照进行抗氧化活性实验,结果表明桑根酮M具有很好的抗氧化活性,其清除DPPH自由基和羟自由基(·OH)的IC50值分别为45.984 mg/L和65.692 mg/L(对照BHT的IC50值分别为64.189、231.556 mg/L),总还原力也明显高于BHT。
A rare flavonoid compound was obtained from black mulberry branches through extraction with 70% cold ethanol, fractionation by organic solvent extraction, column chromatography and thin-layer chromatography（TLC）. The flavonoid was identified as sanggenon M by spectroscopic and structural analysis. Antioxidant activity of sanggenon M was evaluated in comparison with that of the synthetic antioxidant butylated hydroxytoluene（BHT）. The IC50 of sanggenon M for scavenging DPPH radical and hydroxyl radicals were 45.984 and 65.692 mg/L, respectively, compared to 64.189 and 231.556 mg/L for BHT. The total reducing power of sanggenon M was obviously higher than that of the control.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第21期37-40,共4页
Food Science
基金
国家现代农业(蚕桑)产业技术体系建设专项(CARS-22-ZJ0503)
中央高校基本科研业务费专项资金项目(XDJK2015C131)
重庆市研究生科研创新项目(CYS2015069)
关键词
药桑枝条
桑根酮M
分离鉴定
黄酮
抗氧化活性
black mulberry branch
sanggenon M
separation and identification
flavonoid
antioxidant activity
