双酚A的毒性作用机制

Toxic effect mechanism of bisphenol A

针对环境污染日益严重的现状,探讨了双酚A(BPA)对小鼠的肝脏毒性和生殖毒性的作用机制.对血浆和肝脏中的关键酶与精子畸形率进行了检测,并利用紫外可见分光光度计观察了肝脏和睾丸的病理改变.结果表明,BPA可以显著升高谷丙转氨酶(GPT)、谷草转氨酶(GOT)、碱性磷酸酶(AKP)和谷胱甘肽S转移酶(GST)的活力、丙二醛(MDA)和一氧化氮(NO)的含量以及精子畸形率.BPA可以显著降低超氧化物歧化酶(SOD)的活力,并改变了肝脏和睾丸组织的正常形态.BPA不仅可以对肝脏造成损伤,还可以透过血睾屏障损害睾丸的支持细胞和间质细胞,从而干扰精子的生长、发育和能量代谢过程,进而对生殖系统造成损伤.

Aiming at the current situation that the environmental pollution has become increasingly serious, the effect mechanisms of bisphenol A （BPA） on the hepatotoxicity and reproductive toxicity of mice were investigated. The key enzymes in the blood plasma and liver as well as the sperm abnormality rate were tested, and the pathological changes of liver and testis were observed with the ultraviolet and visible spectrophotometer. The results show that the BPA can significantly raise the vitalities of glutamic-pyruvic transaminase （GPT）, glutamic-oxaloacetic transaminase （GOT）, alkaline phosphatase （AKP） and glutathione-S-transferase （GST）, the contents of both malonaldehyde （MDA） and nitrogen oxide （NO） as well as the sperm abnormality rate. In addition, the BPA can obviously reduce the superoxide dismutase （SOD）, and change the normal microstructure morphologies of liver and testis. Therefore, BPA can not only injure the liver, but also damage the supporting and Leydig＇ s cells through penetrating the blood testis barrier to disturb the growth, development and energy metabolism processes of sperms, which will cause the damage to the reproductive system.