miR-29a在系统性红斑狼疮患者血浆中的表达及意义

Expression of miR-29a in plasma of patients with systemic lupus erythematosus and its clinical significance

目的 分析microRNA-29a（miR-29a）在系统性红斑狼疮（SLE）患者血浆中的表达水平及与SLE临床特征的相关性.方法 建立检测miR-29a的实时荧光定量PCR（RT-qPCR）方法,分析48例SLE患者和20例健康体检者血浆中miR-29a的表达水平,ROC曲线评估其作为SLE诊断标记物的意义,统计分析其表达与SLE患者临床特征的相关性.结果 与健康体检者比较,SLE患者血浆miR-29a表达水平明显上调（Z=3.476,P=0.0009）;血清miR-29a诊断SLE的ROC AUC为0.794 （95％Cl：0.689～0.898）,具较好诊断SLE的效能;抗β 2GP1抗体＞20RU/ml患者的血浆miR-29a水平明显升高（P=0.033）,Pearson相关分析显示,SLE患者血浆miR-29a表达水平与抗β2GP1抗体水平呈正相关（r=0.584,P=0.001）,而与不同SLEDAI评分及其他SLE活动相关指标等差异均无统计学意义（均P ＞0.05）. 结论 miR-29a在SLE患者血浆中的表达水平明显上调,且血浆miR-29a上调与SLE患者自身抗体水平相关,其调节机制尚待进一步的研究.

Objective To investigate the expression of microRNA-29a （miR-29a） in plasma of patients with systemic lupus erythematosus（SLE） and its clinical significance. Methods The expression of plasma miR-29a was detected with real-time quantitative polymerase chain reaction（qRT-PCR ）in 48 SLE patients and 20 healthy subjects. The value of miR-29a for diagnosis of SLE was assessed by the receiver operating characteristic （ROC） curve. The relationship between the plasma miR-29a levels and clinical characteristics was analyzed. Results Compared with the healthy controls, the expression of miR-29a in plasma of patients with SLE was significantly higher （Z=3.476, P=0.0009）. The area under the curve （AUC） of ROC was 0.794 （95%CI： 0.689-0.898） . Patients with positive anti- β 2GP1 antibody showed higher miR-29a levels in plasma than those with negative anti- β 2GP1 antibody （P =0.033）. In addition, Pearson analysis indicated that the plasma level of miR-29a was positively correlated with the level of anti- β2GP1 antibodies （r=0.584, P=0.001）. However, the SLEDAI score and other clinical indicators of SLE activation were not significantly correlated with the plasma level of miR-29a. Conclusion The expression of miR-29a in the plasma of SLE patients is up-regulated, which were associated with the levels of several auto-antibodies.