潜伏感染期猪伪狂犬病病毒基因组甲基化预测及图谱测定

Prediction and mapping analysis for the methylation of porcine pseudorabies virus genome during latent infection

为研究DNA甲基化在PRV潜伏感染中的作用,本研究首先对PRV全基因组甲基化状态进行预测,再采用亚硫酸氢盐PCR测序的方法检测急性期及潜伏感染期IE180及EP0启动子区及转录起始位点附近区域的甲基化状态。结果显示,急性期及潜伏感染期IE180及EP0启动子区及转录起始位点附近区域均存在散在的甲基化位点,并未发现广泛的甲基化区域,与预测结果一致。结果表明,潜伏感染期间PRV基因组发生甲基化的可能性很小,进而可知DNA甲基化在PRV潜伏感染过程中未起到关键的作用。

To research the role of DNA methylation in PRV latent infection,this study firstly predicted methylation status of the PRV genome,then bisulfite PCR sequencing was used to detect methylation status of IE180 and EP0promoter and the adjacent transcription start site region during acute and latent infection.The result shows that IE180 and EP0promoter and the adjacent transcription start site region during acute and latent infection are methylated in scattered,and the wide methylated region was not detected,consistent with the prediction.Preliminary study shows PRV is less likely to egtab lish latent infection by DNA methylation,which indicates that DNA methylation may not play a key role during PRV latent infection.