RP-HPLC同时测定人参总皂苷粗提物中人参皂苷Re和Rh_2

Determination of ginsenoside Re and Rh_2 in ginsen raw extraction by RP-HPLC

利用反相-高效液相色谱法（RP-HPLC）建立了快速测定人参皂苷Re和Rh2含量方法。色谱柱为Agilent Extend C18（4.6×150 mm,5μm）,流动相为乙腈-0.05%乙酸梯度洗脱溶液,流速1.00 m L/min,波长203 nm,柱温30.0℃。人参皂苷Re和Rh2的线性范围分别为0.17～456.6 mg/L和6.00～449.4 mg/L;人参皂苷Re和人参皂苷Rh2的平均加标回收率分别为99.60%和99.78%。本方法快速、灵敏、准确性好,可用于测定人参和西洋参提取物中人参皂苷Re和人参皂苷Rh2的含量。

A method for determination ginsedoside Re and Rh2 has been developed by RP-HPLC. Agilent Extend C18（4.6 mm× 150 mm, 5 μm）served as the stationary phase. The mobile phase consists of actonitrile and 0.05% acetic acid in term of gradient operation. Detection wavelength was 203 nrn, column temperature 30.0 ℃, flow rate, 1.00 mL/min. The analytical linearity range of ginsenoside Re and Rh2 were 0.17～456.6 mglL and 6.00-449.4 mg/L, respectively .The recoveries of ginsenoside Re and Rh2 were 99.60% and 99.78%, respectively. The method developed is rapid, simple and accurate and can be used to determine the ginsenoside Re and Rh2 of Radix et Rhizoma Ginseng and Panax quinquefolium L extraction product.