大环内酯类药物对木糖葡萄球菌生物被膜形成的影响

Effects of the intervention biofilm formation of macrolide antibiotics against Staphylococcus xylosus

为探索大环内酯类抗生素是否具有干预木糖葡萄球菌生物被膜形成的能力,采用微量稀释法确定大环内酯类药物红霉素、阿奇霉素以及泰乐菌素对木糖葡萄球菌的最小抑菌浓度,结果分别为0.8、1.6和0.8μg/mL。另外,通过结晶紫染色法、XTT法和LIVE/DEAD BacLight Bacterial Viability Kit染色方法考察了这3种药物对生物被膜的干预作用。结果显示,0.4μg/mL红霉素、0.8μg/mL阿奇霉素和0.4μg/mL泰乐菌素均对木糖葡萄球菌生物被膜的形成能力有干预作用,并且在不同的木糖葡萄球菌菌株呈现出相同的趋势。上述结果表明,大环内酯类药物(红霉素、阿奇霉素和泰乐菌素)能有效地干预木糖葡萄球菌生物被膜的形成。

This experiment is designed to study whether or not macrolide antibiotics can inhibit biofilm formation of Staphylococcus xylosus.By microdilution method,the MICs of erythromycin,azithromycin and tylosin are 0.8,1.6and 0.8μg/mL,respectively.We observed the ability in inhibiting the biofilm formation of S.xylosus by the Crystal violet staining method,XTT and LIVE/DEAD BacLight Bacterial Viability Kit.In result,the three kinds of antibiotics in sub bacteriostatic concentration can interfere with biofilm formation of S.xylosus.We also find the same trendency in different strains of S.xylosus.In conclusion,the experiment has proved that the macrolide antibiotics（erythromycin,azithromycin and tylosin）can effectively inhibit the biofilm formation of S.xylosus.