低温和植物生长物质对南荻种子萌发的影响

Influence of Low Temperature and Plant Growth Substance on Seed Germination of Miscanthus lutarioriparius

以南荻种子为材料,设4℃低温处理(常温为CK)和GA3、IAA、6–BA和2,4–D 4种植物生长物质(各设4个质量浓度50、100、200、400 mg/L),均以清水为CK,处理南荻种子,测定南荻种子的发芽率、发芽势、发芽指数、活力指数、根长以及芽长等指标。结果表明,与CK相比,4℃低温处理能显著提高南荻种子的发芽率、发芽势,极显著提高南荻种子的发芽指数、活力指数、根长,但芽长差异没有统计学意义;各质量浓度的GA3能显著或极显著提高发芽率、发芽势、发芽指数、活力指数和芽长,其中100 mg/L GA3处理对南荻种子萌发的效果较好;除400 mg/L外,IAA各质量浓度均能提高发芽率、发芽势、活力指数和促进根的生长,其中以50 mg/L IAA处理对南荻种子的萌发效果较好;各质量浓度的6–BA处理能极显著提高南荻种子的发芽率、发芽指数,显著或极显著提高南荻种子的发芽势和促进芽的生长,极显著的降低南荻种子的活力指数和抑制根的生长;2,4–D对南荻种子发芽率、发芽势以及发芽指数的影响都随浓度的增加先升高后下降,各质量浓度的2,4–D可极显著降低南荻种子的活力指数和抑制根的生长,其中以200 mg/L 2,4–D处理对南荻种子萌发的效果较好。

Two temperature treatments （4 ℃, room temperature for CK） and four plant growth substances of GA3, IAA, 6-BA and 2,4-D （ each of them had four mass concentrations of 50, 100, 200, 100 mg/L, take water as CK） were designed to study the effects of low temperature and plant growth substance on the seed germination of Miscanthus lutarioriparius.The results were as the follow： Compared with CK, 4 ℃ treatment could significantly improve the seed germination rate, germination potential and extremely significantly improve the germination index, vigor index and root length, but did not significantly improve bud length; All mass concentration of GA3 could significantly improve the germination rate, germination potential, germination index, vigor index and bud length, which the concentration of 100 mg/L had the most effect on germination; except 400 mg/L concentration, the other concentrations of IAA could improve the germination rate, germination potential, vigor index and promote root growth, the concentration of 50 mg/L was the best among them; The mass concentration of 6-BA could significantly improve the germination rate, germination index, germination potential and bud growth, but significantly reduce seed vigor index and the root growth; the effects of 2,4-D on germination rate, germination potential and germination index were rise first then falling down with the increase of the concentration, all mass concentration of 2,4-D could extremely significantly reduce vigor index and the root growth, 200 mg/L concentration was the best among them.