用于肿瘤治疗的小分子干扰RNA非病毒载体研究进展

Current status of non-viral siRNA vectors for therapy of cancers

近年来,小分子干扰RNA(siRNA)作为RNA干扰(RNAi)技术的效应分子,已被广泛用于恶性肿瘤的基因治疗领域。欲获得理想的治疗效果,其关键因素是寻找一种安全、高效、稳定、可控的基因载体。非病毒载体具有低毒、低免疫原性、制备简单、目的基因容量大、外源基因随机整合率低且携带基因大小类型不受限制等突出优势,已经成为目前siRNA载体的研究热点。在以往学者的研究基础上,从药剂学的角度,笔者对这些载体在siRNA传递系统中的研究现况做回顾性总结。

In the recent years ,siRNA has been widely used as effector molecule in the field of gene therapy for cancer . In order to achieve ideal treatment effect ,the key factor is to find safe ,efficient ,stable and controllable gene vectors .The commonly used vectors include viral and non-viral vectors .The non-viral gene vector has superiority of low toxicity ,bare im-munogenicity ,simple to manufacture and high capacity which have been taken as the highlight of the research of carrier of gene drugs .Based on the study of the previous researchers ,this study reviewed the prevalence study of the former carriers in the de-livery system of siRNA from the point of pharmaceutics .