应用高效液相色谱法测定消亢胶囊中迷迭香酸的含量

Determination of the rosmarinic acid in Xiaokang capsule by HPLC

目的：建立测定消亢胶囊含量的方法。方法采用高效液相色谱（HPLC）法测定消亢胶囊中迷迭香酸的含量。色谱柱：Agilent C18柱，流动相：甲醇-0．5％甲酸（50∶50），流速：1 ml／min ，检测波长：330 nm ，柱温：30℃，进样量：10μl。结果消亢胶囊中夏枯草迷迭香酸的浓度在2．88～20．16μg范围内与峰面积积分值呈良好的线性关系（ r＝0．9998），平均回收率为99．54％，RSD为0．40％（n＝9）。结论该方法操作简便、结果准确可靠，适用于消亢胶囊中迷迭香酸的含量测定。

Objective To develop a method for determination of the rosmarinic acid in Xiaokang capsules .Methods An HPLC method was developed .The determination was performed on Agilent C18 column at 30℃ with a mobile phase composed of methanol ：0 .5% formic acid （50∶50） run at a flow rate of 1 .0 ml/min .The detection wavelength was set at 330 nm and the injection volume was 10 μl .Results The linear range of rosmarinic acid concentrations was 2 .88 to 20 .1μg/ml （r=0 .999 8） . The average recovery was 99 .5% （RSD=0 .40% ,r=9） .Conclusion An assay for the determination of the rosmarinic acid in Xiaokang capsule that is simple ,rapid ,accurate and reliable has been developed .