右归饮促异体骨髓细胞向成骨分化防治辐照后骨量丢失机制的实验研究

Experiment mechanism study on bone loss prevention caused by irradiation with Youguiyin promoted the osteogenic differentiation of the allogeneic bone marrow stem cell

目的:研究右归饮调控辐射后小鼠体内的异体骨髓细胞向成骨细胞分化,尝试揭示其防治辐照引起的骨量丢失的机制。方法:选用80只8 w龄雄性BALB/c小鼠,随机选取32只用于骨髓细胞提取和回输。将剩余48只小鼠随机分成6组,每组8只,分别为空白对照组、辐照组、骨髓细胞(BM)回输组、右归饮高剂量组、中剂量组、低剂量组。辐照组:接受6.0 Gy的γ辐照;BM回输组:6.0 Gy的γ辐照+同种异体骨髓细胞1∶1回输;右归饮高、中、低剂量组:在骨髓回输组的基础上,分别将0.51 g/m L浓度的右归饮每天以0.03 g/m L、0.02 g/m L、0.01 g/m L灌胃。2 w后,检测各组小鼠血清碱性磷酸酶(ALP)、双侧股骨单位体积内骨量(即骨密度)的差异及股骨组织病理变化。结果:右归饮高剂量组双侧股骨骨密度显著高于辐照组、BM回输组(P<0.01),右归饮中剂量组与辐照组、BM回输组比较差异均具有统计学意义(P<0.05);右归饮高、中剂量组及空白对照组ALP浓度显著高于辐照组(P<0.01),且高剂量组高于低剂量组、BM回输组(P<0.05)。组织病理显示:辐照后骨髓组织结构凌乱,骨髓单核细胞大量消失,部分组织变性,骨小梁稀疏甚至消失,经BM回输后骨小梁重现,骨髓单核细胞数量明显恢复,与BM回输组比较,高剂量组骨小梁排列有序坚实,脂肪空泡数量少,接近空白对照组。结论:右归饮可促进异体骨髓细胞向成骨分化,改善辐照后小鼠骨量丢失情况。

Objective：To study the influence of Youguiyin on the osteogenic differentiation of the allogeneic bone marrow stem cell in mice so as to reveal the mechanism of Youguiyin preventing bone loss caused by irradiation. Methods：Eighty BALB/c male mice with age of 8-week were prepared. 32 mice of them were used to extract bone marrow stem cell for transplantation. 48 mice were randomly divided into control group,irradiation group,bone marrow cell（BM）transplanting group,Youguiyin high dose group,middle dose group,low dose group. The mice in irradiation group were exposed to 6.0 Gy gamma irradiation. The mice in BM transplanting group were exposed 6.0 Gy irradiation and received bone marrow cells. The mice in high dose group,middle dose group and low dose group were exposed to 6.0 Gy irradiation and received bone marrow cells and gavaged with Youguiyin of 0.03 mL/g,0.02 mL/g,0.01 mL/g,respectively. After 2 w,serum alkaline phosphatase （ALP）,bone volume of femur bone （bone mineral density）and pathological tissue changing were observed. Results：Compared with the irradiation group and BM transplanting group,bone mineral density in high dose group was significantly increased （P〈0.01）. Middle dose group had a statistically difference with the irradiation group and BM transplanting （P 〈 0.05）.Compared with the irradiation group,in high does group,middle dose group and control group ALP had increased （P〈0.01）and high does group had a statistically difference with low dose group and BM transplanting group （P〈0.05）. Femur pathology：After irradiating,the structure of myeloid tissue become mass and monocyte was rare in the bone marrow and a part of tissue were degenerated. Bone trabeculas reappeared and bone marrow mononuclear cells were recovered,when injected with bone marrow stem cell. In addition,high dose group has strong and well ordered trabecular,less fat cavitations. Conclusion：Youguiyin can promote the osteogenic differentiation of the allogeneic bone marrow stem cell to cure the bone loss caused by irradiation.