星点及正交实验法优选穿龙薯蓣总皂苷的提取工艺

Study on optimum process of total sapogenin extracted from dioscorea nipponica based on central composite design and orthogonal experimental design

目的:用星点与正交设计两种方法优化穿龙薯蓣总皂苷的提取工艺。方法:采用回流法提取穿龙薯蓣总皂苷,以薯蓣皂苷为对照品,以乙醇浓度、提取时间、提取温度和料液比为考察指标,通过UV法测定其总皂苷含量。结果:最终确定穿龙薯蓣总皂苷的最佳提取工艺为:乙醇浓度为75%,料液比为16,提取时间为130 min/次,提取温度为85℃。结论:该方法设计精确,数据可靠,可为穿龙薯蓣总皂苷的提取提供了理论依据。

Objective：To optimize the process of total sapogenin extracted from dioscorea nipponica makino with central composite design and orthogonal experimental design. Methods：The refluxing extraction method of total sapogenin was adopted,dioscin as reference substance,with ethanol concentration,extraction time,extraction temperature and solid-liquid ratio as examining indice. The content of sapogenin was measured by UV method. Results：The optimum extraction conditions of sapogenin were as follows,75% ethanol concentration,solid-liquid ratio was 16,the extraction time was 130 min per time,extraction temperature was 85 ℃. Conclusion：This experimental design method is precise and the data is reliable. It can provide a theoretical basis for the extraction of total sapogenin extracted from dioscorea nipponica makino.