KLKs基因表达对卵巢癌恶性度评估初探

Evaluation of the Malignant Degree of Ovarian Cancer by KLKs Gene Expression

目的研究激肽释放酶(kallkarein)KLK10及KLK11在卵巢癌组织中的表达。方法应用IHC技术检测18例正常卵巢组织、32例良性卵巢肿瘤以及43例卵巢癌组织中KLK10,KLK11的hkn蛋白表达。结果 KLK11在健康卵巢、卵巢良性肿瘤、卵巢癌上皮组织细胞中阳性率分别为77.7%、78.1%、53.5%;KLK10分别为83.3%、78.1%、46.5%;Klk11于卵巢癌组织中的表达比正常的卵巢组织和良性的卵巢肿瘤组织表达的水平要低,差异有统计学意义(P<0.05);Klk10于卵巢癌组织中的表达比其在正常的卵巢组织和良性的卵巢肿瘤组织表达的水平要低,差异有统计学意义(P<0.05)。结论卵巢癌的组织中一般存在KLKll和KLK10表达水平的下调,表明卵巢癌当中KLKH的表达失调,这一点对卵巢癌的临床诊断以及预后的意义深远。

Objective To study the expression of kallikrein KLK10 and KLK11 in tissues of ovarian cancer. Methods IHC technique was used to detect the expression of KLK10 and KLK11 hkn proteins in 18 specimens of normal ovarian tissues, 32 specimens of benign ovarian tumor tissues and 43 specimens of ovarian cancer tissues. Results The positive rate of KLK11 in healthy ovarian tissues, benign ovarian tumor tissues, ovarian cancer epithelial cells was 77.7%, 78.1%, 53.5%, respectively; and that of KLK10 in the above tissues was 83.3%, 78.1%, 46.5%, respectively. The expression of KLKll in ovarian cancer tissues was lower than that in healthy ovarian tissues and benign ovarian tumor tissues, the difference was statistically significant （P〈0.05）; the expression of KLK10 in ovarian cancer tissues was lower than that in healthy ovarian tissues and benign ovarian tumor tissues, the difference was statistically significant （P〈0.05）. Conclusion The expression of KLKll and KLK10 genes in the ovarian cancer tissues is downregulated generally, indicating that the expression of KLKH gene in the ovarian cancer tissue is imbalanced, which is of great significance in the clinical diagnosis and evaluation of the prognosis of ovarian cancer.