利斯的明中间体含量及ee值的RP-HPLC法测定

Determination of ee values and content of rivastigmine intermediates by RP-HPLC

目的:建立RP-HPLC法测定生物转化液中利斯的明中间体含量及ee值的方法。方法:以Lux 3u Cellulose-1为手性分离柱,以正己烷∶异丙醇(V∶V=92.5∶7.5)为流动相,检测波长217 nm,进样量20μL。结果:中间体能够完全分离,在测定中间体含量的同时能够测定样品的ee值。结论:该方法准确、灵敏、精密度良好,适合生物转化液中利斯的明中间体含量及ee值的测定。

Aim：To establish a RP-HPLC method to determine the ee values and content of rivastigmine intermediates in bioconversion broth.Methods：The determination was performed on a Lux 3u Cellulose-1 column with a 92.5：7.5（V：V） mixture of n-hexane and isopropanol alcohol as mobile phase , determination wavelength of 217 nm and injection volume of 20 μL.Results：The two intermediates were completely separated .The ee value could be determined as well as the de-termination of the content of the intermediates .Conclusion：The method is accurate and sensitive with high precision .It is suitable for the determination of the ee values and content of rivastigmine intermediates in bioconversion broth .