不同活血化瘀方对扩张型心肌病小鼠心肌细胞的作用及对NF-κB通路的影响

Effects of different activating blood and removing stasis prescriptions on myocardial cells of mice with dilated cardiomyopathy and NF-κB pathway

目的:研究不同活血化瘀方对扩张型心肌病小鼠心肌细胞的作用及对NF-κB通路的影响。方法:240只BALB/c小鼠随机分为方剂A组、方剂B组、模型组和空白组,每组60只。前3组采用柯萨奇B3m病毒制作实验室病毒性扩张型心肌病模型,方剂A组、方剂B组小鼠造模后分别以方剂A和方剂B灌胃治疗,模型组和空白组小鼠均灌注等量的纯水,1次/d,连续5周。比较各组小鼠的心脏质量指数。运用HE和VG染色法观察各组小鼠心肌细胞的病理学改变。采用TUNEL法检测小鼠心肌细胞的凋亡情况。采取RT-PCR法和免疫组化法检测各组小鼠心肌细胞NF-κB mRNA和蛋白的表达情况。结果:方剂A组、方剂B组和模型组小鼠心脏质量指数、心肌胶原容积分数、凋亡心肌细胞数及NF-κB mRNA和蛋白的表达均高于空白组(P<0.05);方剂A组和方剂B组小鼠的上述5个指标均低于模型组(P<0.05);方剂A组上述5个指标均低于方剂B组(P<0.05)。结论:不同活血化瘀方对扩张型心肌病小鼠心肌细胞的改善作用和对NF-κB通路的影响有差异。

Aim：To study the effect of different promoting blood circulation to remove blood stasis prescriptions on myocardial cells of mice with dilated cardiomyopathy and the effect on the NF-κB pathway .Methods： A total of 240 BALB/c mice were allocated into 4 groups with 60 in each group：group A,group B,model group,and control group.The model of dilated cardiomyopathy was made by B 3m virus in the first 3 groups.The model mice in group A and group B were treated by two kinds of activating blood and removing blood stasis decoction .The heart mass index of the mice was com-pared .The pathological changes of myocardial cells from the mice were observed by HE and VG staining .The apoptosis of myocardial cells was detected by TUNEL .The expressions of NF-κB protein and mRNA were detected by immunohisto-chemical method and RT-PCR.Results：The heart mass index , myocardial collagen volume fraction , apoptosis of myocar-dial cells, and expressions of NF-κB protein and mRNA of group A , group B and the model group were higher than the con-trol group（P〈0.05）.The 5 indexes of group A and group B were lower than those in the model group （P〈0.05）.And the 5 indexes of group A were lower than those in group B （P〈0.05）.Conclusion：The effects of different promoting blood circulation to remove blood stasis prescriptions on myocardial cells of mice with dilated cardiomyopathy and the effect on the NF-κB pathway are different .