脑动静脉畸形病变血管组织学变化和血管内皮生长因子的表达

Histological changes and expression of VEGF in cerebral arteriovenous malformation vascular tissue

目的:探索脑动静脉畸形(CAVM)与脑血管内皮生长因子(VEGF)之间的关系。方法:对30例CAVM病变血管及病变周围血管组织标本行HE染色,观察血管的组织学改变。采用免疫组化法和Western blot法检测血管中VEGF蛋白的表达,采用荧光定量PCR法检测血管中VEGF mRNA的表达。以32例正常脑组织血管标本作对照。结果:CAVM病变血管和病变周围血管较正常脑血管发生明显改变。荧光定量PCR和Western blot检测结果显示,CAVM病变血管和病变周围血管组织中VEGF mRNA和蛋白表达水平均高于正常脑血管(P均<0.05),CAVM病变血管中VEGF mRNA和蛋白表达水平高于病变周围血管(t配对=19.095,3.216,P<0.05)。结论:VEGF可能对CAVM的发生和发展起着重要作用。

Aim：To explore the pathological characteristics of cerebral arteriovenous malformation （ CAVM） and the relationship between CAVM and vascular endothelial growth factor （VEGF）.Methods： A total of 30 CAVM and the pe-ripheral vascular tissue were collected .Histological changes in vascular tissues were observed using HE staining .The ex-pression of VEGF proteins were detected by immunohistochemistry and Western Blot .The expression of VEGF mRNA were detected by Real-time PCR.32 normal brain vascular tissue were the control .Results： The histological characteristics of CAVM and peripheral vascular tissue were obviously different from the normal brain vascular tissue .The results of Real-time PCR and Western blot showed that the expression of VEGF protein and mRNA in CAVM and peripheral vascular tissue were significantly higher than those in normal brain vascular tissue （P〈0.05）, and the expression of VEGF protein and mRNA in CAVM were higher than those in peripheral vascular tissue （tmatch =19.095,3.216,P〈0.05）.Conclusion：VEGF may play an important role in the occurrence and development of CAVM .