TEKT3在特发性弱精子症患者精子中的表达

Expression of TEKT3 in spermatozoa of idiopathic asthenozoospermia patients

目的:探讨特发性弱精子症患者精子中TEKT3表达的特点。方法:采用非连续密度梯度离心法分离、纯化正常对照组和弱精子症组精子标本各35份,分别采用RT-PCR和Western blot检测精子中TEKT3 mRNA和蛋白的表达。结果:与正常对照组比较,弱精子症组TEKT3 mRNA和蛋白表达均显著降低(t=9.610、10.090,P<0.001),相关性分析显示弱精子症组精子TEKT3 mRNA和蛋白表达水平与前向运动精子率呈正相关(r=0.684,P=0.001;r=0.483,P=0.013)。结论:TEKT3表达降低可能在特发性弱精子症发病中具有重要作用。

Aim：To investigate the function of TEKT 3 protein in the pathogenesis of patients with idiopathic astheno-zoospermia .Methods：Semen samples were collected from 35 normal controls and 35 idiopathic asthenozoospermia patients and purified using Percoll discontinuous density gradients , then the expression of TEKT 3 mRNA and TEKT3 protein were detected by RT-PCR and Western blot .Results： Compared with normal controls , the expressions of TEKT3 mRNA and protein in spermatozoa of idiopathic asthenozoospermia group were obviously decreased （t=9.610,10.090,P〈0.001）. Correlation analysis revealed that the expressions of TEKT 3 mRNA and protein were positively correlated with spermatozoa progressive motility（r=0.684,P=0.001;r=0.483,P=0.013）.Conclusion： The reduced expression of TEKT3 may play an important role in the pathogenesis of idiopathic asthenozoospermia .