摘要
背景:椎体终板由椎体的骨骺骨化形成,脊柱侧凸畸形导致椎体终板承受不正常的应力,此病理情况下椎体终板结构的改变尚未完全明确。目的:使用micro CT对大鼠不对称力脊柱侧凸模型椎体终板结构的变化进行观察。方法:选取5周龄雌性SD大鼠15只,手术将钛钉由大鼠L2、L5右侧横突根部置入椎体,并连结镍钛弹簧建立腰椎不对称力脊柱侧凸模型。7周后使用micro CT对脊柱侧凸顶点处椎体终板进行扫描分析,Mimics软件对椎体终板骨性结构及内部孔道结构进行3D重建。结果:7周后大鼠脊柱腰段侧凸Cobb角由术后即刻平均25.7°±2.4°进展至平均61.5°±5.4°,micro CT平扫重建数据显示脊柱侧凸的凹侧椎体终板较凸侧明显变薄,其内部孔道结构稀疏,骨体积分数(BV/TV)明显增加。结论:腰椎不对称力导致脊柱侧凸的凹侧椎体终板趋向形成一致密的薄骨板,这种病理改变可能对凹侧椎体的生长及椎间盘的营养产生不利影响。
Background： The vertebral endptate is formed by epiphyseal ossification of the vertebral body. Abnormal mechanical loading appears in scoliosis as compared to normal spines. How the vertebral endplate was changed in scoliosis is not well understood. Objective： To determine structural changes of vertebral endplate in asymmetric load scoliosis rat models by microCT. Methods： Fifteen 5-week-old female Sprague-Dawley rats were selected to develop lumbar scoliosis by using a nickel-titanium （NT） coil spring. Two bone screws were implanted in vertebral bodies via the roots of right transverse processes of L2 and L5. The two ends of NT coil spring attached to L2 and L5 screws. MicroCT was used to analyze the vertebral endplate at the curve apex in rat lumbar scoliosis models after 7 weeks and the 3D models of the endplate bone structure and the internal canal structure were reconstructed by Mimics software. Results： The average initial coronal Cobb angle was 25.7°±2.4° immediately after the operation and increased to 61.5°±5.4° on average over 7 weeks. The 3D models showed that the concave side of the scoliotic endplate was thinner than the convex side, the structure of canals was sparse, and the bone volume fraction significantly increased. Conclusions： The concave side of the vertebral endplate tends to form a thin dense bony plate under lumbar asymmetric load. Such pathological changes may adversely affect the growth of concave side of the vertebrae and the intervertebral disc nutrition.
出处
《中国骨与关节外科》
2015年第5期429-433,共5页
Chinese Journal of Bone and Joint Surgery
基金
国家自然科学基金资助项目(81000817)
