摘要
应用ISSR技术对有棱丝瓜[Luffa acutangula(L.)Roxb.]与普通丝瓜[Luffa cylindrica(L.)Roem.]及其种间杂种F1、F2代的遗传差异性进行了研究。结果表明,供试材料基因组DNA间具有很高的多态性,双亲的遗传相似系数仅为0.395,F_1的条带主要表现为双亲互补带型,F_1、F_2并没有明显向双亲任何一方遗传的倾向,F_2的遗传变异较大。
The genetic differences among Luffa acutangula (L.) Roxb., Luffa cylindnca (L.) Roem. and their F1 hybrid and F2 population were analyzed by ISSR technolo- gy. The results showed that the genomic DNA of test materials showed a high diversity; the genetic similarity coefficient between both parents was only 0.395; the bands of F1 hybrid mainly consisted of both parents'; neither FI nor F2 was inherit- ed singly from male or female parent; there was greater genetic variation among F2 population.
基金
Supported by Jiangsu Province Science and Technology Support Program,China(BE2012323)
Jiangsu Agricultural Science and Technology Innovation Fund[CX(11)1002]~~
关键词
有棱丝瓜
普通丝瓜
种间杂种
ISSR
Luffa acutangula (L.) Roxb.
Luffa cylinddca (L.) Roem.
Interspecific hybrid
ISSR