Ag纳米粒子表面BSA隔离层对聚苯乙烯荧光的增强效应

Fluorescence enhancement effect of polystyrene by BSA isolation layer at surface of Ag nanoparticles

通过氧化还原法制备了粒径处于50-65nm的球形银（Ag）纳米粒子,采用扫描电子显微镜（SEM）分析其形貌及单分散性.在Ag纳米粒子基础上采用沉积自组装法合成了有效粒径为80.8nm 的Ag/牛血清白蛋白（BSA）核壳结构纳米粒子. 结合SEM、透射电子显微镜（TEM）、X 射线衍射（XRD）和荧光发射光谱（FL）分析发现,BSA 有效地包覆在Ag 纳米粒子的外层,Ag/BSA 核壳结构纳米粒子单分散性良好,加入Ag/BSA 核壳结构纳米粒子的聚苯乙烯（PS）的荧光强度从100增强到6000.研究结果表明,BSA隔离层对位于Ag表面附近的PS分子的荧光强度有显著的增强效应.

Spherical silver(Ag)nanoparticles with the average diameter 50-65 nm were synthesized by oxidationreduction process.The morphology and monodispersity of the Ag nanoparticles was characterized by scanning electron microscope(SEM).Ag/bovine serum albumin(BSA)core-shell structure nanoparticles approximately80.8nm in effective diameter were fabricated by deposition method and self-assembling onto Ag nanoparticles.The results of SEM,transmission electron microscope(TEM),X-ray diffraction(XRD)and fluorescence emission spectra(FL)showed that BSA effectively coated on the surface of Ag nanoparticles,and Ag/BSA coreshell structured nanoparticles had good monodispersity.Furthermore,fluorescence intensity of PS enhanced from 100 to 6 000 by Ag/BSA core-shell structured nanoparticles.The results showed that BSA isolation layer had significant enhancement effect on fluorescence intensity of PS near the surface of Ag.