云南地方晾晒烟化学成分研究

Study on Chemical Ingredients of Air-cured Tobacco in Yunnan Province

为研究云南大理云龙天登晾晒烟的化学成分,将烟叶用95%乙醇提取、浓缩,对浓缩液用乙酸乙酯萃取,将乙酸乙酯萃取部分进行系统化学成分分离,采用正相硅胶柱色谱及MCI,PLgel,Sephadex LH-20,C18反相半制备高效液相色谱等柱色谱方法进行分离,根据理化性质和波谱数据对其结构鉴定.结果表明,从乙酸乙酯部分得到7个化合物,分别为7-Hydroxy-6-methoxy-2H-1-benzopyran-2-one Scopoletin(1),(7S,8R)-dihydrodehydrodiconiferyl alcohol(2),N-trans-feruloyltyramine(3),N-cis-feruloyltyramine(4),香草酸(5),对羟基苯乙醇(6),3,4-二羟基苯甲酸(7).其中除化合物5外,其余6个化合物均为首次从该烟草中分离鉴定.

To study the chemical constituents of Air-cured tobacco selected from Yunlong Tianden Dali,Yunnan province,95% ethanol was used to extract the tobacco leaf;the ethyl acetate part was isolated and purified by repeated column chromatography on silica gel, MCI,PLgel,Sephadex LH-20,C1 8 HPLC.The structures of chemical constituents were identified according to the physicochemical prop-erty and spectral data of compounds.The result showed that seven compounds were isolated from the ethyl acetate part identified as 7-Hydroxy-6-methoxy-2H-1 -benzopyran-2-oneScopoletin(1 ),(7S,8R)-dihydrodehydrodiconiferyl alcohol(2),N-trans-feruloyltyramine (3),N-cis-feruloyltyramine (4),vanillic acid (5),tyrosol(6),protocatechuic acid(7).Among them except No.5,other six ones are the first to be isolated and identified from the tobacco.