乙型肝炎肝硬化患者外周血IFN-γ、IL-32和IL-6的临床相关性

Clinical correlation with serum IFN-γ,IL-32 and IL-6 levels in patients with HBV associated liver cirrhosis

目的探讨乙肝肝硬化患者外周血IFN-γ、IL-32和IL-6水平的临床相关性。方法收集42例乙型肝炎肝硬化患者和50例健康对照者空腹血清,采用ELISA法检测血清IFN-γ、IL-32和IL-6水平;采用荧光定量PCR法检测血清HBV DNA载量;采用全自动生化分析仪检测血清肝功能水平。结果健康对照者血清IFN-γ、IL-32和IL-6水平分别为(14.41±3.71)、(182.24±72.22)、(1.62±0.63)ng/mL,乙肝肝硬化患者分别为(182.12±41.37)、(1218.21±215.08)、(37.70±9.77)ng/mL。IFN-γ水平与ALb水平呈负相关(r=-0.342,P<0.05),与HBV DNA水平呈正相关(r=0.319,P<0.05);IL-6水平与ALT水平呈负相关(r=-0.314,P<0.05)。结论 IFN-γ、IL-32和IL-6在慢性HBV感染过程中可能起重要作用,可作为评估慢性乙型肝炎病情严重程度的重要指标。

Objective To investigate the clinical correlation with serum levels of interferon‐gamma （IFN‐γ） , interleukin （IL ） 32 and IL‐6 in patients with hepatitis B virus （HBV ） associated liver cirrhosis .Methods Forty‐two patients with HBV associated liver cirrhosis and 50 healthy controls were enrolled to collect their fasting serum samples . Serum IFN‐γ ,IL‐32 and IL‐6 levels were detected by enzyme‐linked immuno sorbent assay （ELISA） .Measurement of HBV DNA load and liver function were carried out by fluorescence quantitative polymerase chain reaction （PCR） and automatic biochemical analyzer respectively to analyze their clinical correlation .Results Compared with those in healthy controls （14 .41 ± 3 .71 ,182 .24 ± 72 .22 ,1 .62 ± 0 .63 ng/mL） ,levels of serum IFN‐γ,IL‐32 and IL‐6 in patients with liver cirrhosis were significantly increased （182 .12 ± 41 .37 ,1218 .21 ± 215 .08 ,37 .70 ± 9 .77 ng/mL） ,respectively .IFN‐γ levels were negatively correlated with ALB levels （r= -0 .342 ,P=0 .027〈0 .05） ,while positively correlated with HBV DNA load （r=0 .319 ,P=0 .039〈0 .05） .There was a negative correlation between IL‐6 levels and ALT levels（r= -0 .314 ,P=0 .043〈0 .05） .Conclusion IFN‐γ,IL‐32 and IL‐6 might play important roles in chronic HBV infection ,serum levels of which could be important indicators to assess the severity of chronic hepatitis B in patients .