摘要
目的探讨子宫内膜异位症不同中医证型与胰岛素样生长因子-Ⅰ的关系。方法选取因子宫内膜异位症需行腹腔镜手术的患者242例,另22例因不孕或者盆腔良性疾病需行腹腔镜手术患者为对照组,采用ELISA检测血清中胰岛素样生长因子-Ⅰ,免疫组化检测内膜组织中胰岛素样生长因子-Ⅰ的表达情况。结果242例子宫内膜异位症患者中气滞血瘀型患者87例(36.0%),痰湿瘀阻型36例(14.9%),寒凝血瘀型33例(13.6%),热郁血瘀型24例(9.9%),肾虚血瘀型43例(17.8%),气虚血瘀型19例(7.8%)。ELISA检测结果显示,气虚血瘀组患者血清中IGF-Ⅰ含量与正常对照组无差异外,其他各组均高于正常对照组(P〈0.05);气滞血瘀组、肾虚血瘀组患者血清中IGF-Ⅰ含量明显高于其他组别(P〈0.05);免疫组化检测结果显示,不同中医证型患者组织IGF-Ⅰ阳性表达率相对于正常对照组具有统计学意义(P〈0.05,P〈0.01),气滞血瘀型组IGF-Ⅰ阳性表达率高于痰湿瘀阻组、热郁血瘀组(P〈0.05),气滞血瘀组在9∽12评分(+++)项中表达高于其他各组(P〈0.05)。结论子宫内膜异位症患者中医证型中气滞血瘀、肾虚血瘀以及寒凝血瘀型与IGF-1的高表达有关。
Objective To investigate the relationship of endometriosis syndrome type and insulin-like growth factor- I. Methods To select 242 cases as for TCM gynecology gynecology or endometriosis patients and 22 cases of laparo- scopic surgery because of infertility or pelvic laparoscopic surgery for benign disease need patients in the control group,using ELISA serum insulin-like growth factor- I , immunohistochemical detection of endometrial tissue expres- sion of insulin-like growth factor- I. Restdts The 242 patients with endometriosis in patients with blood stasis type 87 (36.0%) ,phlegm stasis type 36 cases (14.9%), Hanning Blood type 33 cases (13.6%) ,hot Yu blood stasis 24 cases (9.9%) ,43 cases of kidney and blood stasis ( 17.8% ) and 19 cases of Qi and Blood (7.8%). ELISA test re- suits showed no difference in serum QDBS content of IGF- I group and the normal control group, the other groups were higher than the normal control group ( P 〈 0.05 ) ; blood stasis group, Kidney Blood patients serum IGF-I were significantly higher than the other groups ( P 〈 0.05 ). Immunohistochemistry showed, IGF- I in patients with positive expression of tissue syndrome type relative to the control group with statistical significance ( P 〈 0.05, P 〈 0. 01 ) ; blood stasis type group IGF-| expression rate higher than phlegm stasis group, heat and blood stasis group ( P 〈 0. 05 ) ; blood stasis group was higher than the other groups (P 〈 0.05 ) in the 9-12 score ( + + + ) entry. Conclusion Endometriosis patients with TCM syndrome type of qi and blood stasis, kidney and blood stasis and blood stasis type haemorrheological nature related to the high expression of IGF-1.
出处
《吉林中医药》
2015年第11期1118-1121,共4页
Jilin Journal of Chinese Medicine
基金
湖北省自然科学基金资助项目(2010CDB09403)
关键词
子宫内膜异位症
胰岛素样生长因子-Ⅰ
气滞血瘀
肾虚血瘀
寒凝血瘀
endometriosis
insulin-like growth factor-I
qi-stagnation and blood stasis
renal deficiency and blood sta-sis
congealing cold and blood stasis